Postprandial lipemia causes oxidative stress in dogs

Author(s)

Minucci Bonatto, Natalia Camila ^[1] ; de Oliveira, Paula Lima ^[2] ; Mancebo, Aline Martins ^[1] ; Costa, Leticia Ramos ^[2] ; Melo Bosculo, Maria Raquel ^[2] ; Bosco, Anelise Maria ^[3] ; Ciarlini, Paulo Cesar ^[3] ; Floriano, Beatriz Perez ^[4] ; de Barros, Luiz Daniel ^[1] ; Martins de Almeida, Breno Fernando ^[4]

Affiliation

^[1] Londrina State Univ UEL, Dept Prevent Vet Med, Londrina, Parana - Brazil

^[2] Univ Ctr Integrated Fac Ourinhos Unifio, Roque Quagliato Vet Hosp, Ourinhos, SP - Brazil

^[3] Sao Paulo State Univ, Coll Vet Med Aracatuba, Dept Clin Surg & Anim Reprod, Aracatuba, SP - Brazil

^[4] Univ Ctr Integrated Fac Ourinhos Unifio, Dept Vet Med, Rodovia BR 153, Km 338, BR-19909100 Ourinhos, SP - Brazil

• Document type: Journal article
• Source: Research in Veterinary Science; v. 136, p. 277-286, MAY 2021.
• Web of Science Citations: 0

Abstract

Oxidative stress (OS) has been strongly associated with postprandial lipemia (PPL) in humans, and still requires further investigation in dogs. However, since lipemia interferes with spectrophotometric determinations such as those used to assess OS, the present study investigated the effect of PPL on OS parameters of healthy dogs. Twenty dogs had lipemic postprandial samples compared to the average of two non-lipemic moments. Subsequently, PPL was simulated in vitro using a commercial lipid emulsion and twelve pools of non-lipemic serum of these dogs were used to simulate the minimum, median and maximum concentrations of triglycerides obtained during the lipemic state. Serum OS parameters were assessed using the antioxidants uric acid, albumin and total bilirubin; total antioxidant capacity (TAC); total oxidant capacity (TOC); and lipid peroxidation. In vivo PPL caused an increase in albumin, TAC-CUPRAC, TAC-FRAP, uric acid (p < 0.0001), TOC (p = 0.0012) and total bilirubin (p = 0.0245); reduction of TAC-ABTS (p = 0.0008); and did not alter the lipid peroxidation (p = 0.8983). In vitro, levels of albumin increased at the three lipemic concentrations (p < 0.0001), uric acid increased in the median and maximum levels (p < 0.0001), and total bilirubin concentration increased only at the maximum lipemic level (p = 0.0012). All lipemic levels tested increased TAC-ABTS (p = 0.0011) and TACFRAP (p < 0.0001). TAC-CUPRAC (p = 0.5002), TOC (p = 0.5938) and lipid peroxidation (p = 0.4235) were not affected by in vitro lipemia. In conclusion, both the in vivo postprandial state and in vitro simulated lipemia affect oxidative stress markers in dogs depending on the oxidative stress marker, and thus the postprandial state and/or lipemic samples should be avoided. (AU)

• FAPESP's process: 18/18220-0 - Effect of postprandial lipemia on canine oxidative stress markers
• Grantee: Breno Fernando Martins de Almeida
• Support type: Regular Research Grants
• FAPESP's process: 18/16334-9 - Effect of postprandial lipemia on canine oxidative stress markers
• Grantee: Natália Camila Minucci Bonatto
• Support type: Scholarships in Brazil - Scientific Initiation