Macrophage Polarization in the Skin Lesion Caused by Neotropical Species of Leishmania sp

Author(s):Show less -

Sandoval Pacheco, Carmen M. ^[1] ; Araujo Flores, V, Gabriela ; Gonzalez, Kadir ^[2] ; de Castro Gomes, Claudia M. ^[3] ; Passero, Luiz F. D. ^{[4, 5]} ; Tomokane, Thaise Y. ^[3] ; Sosa-Ochoa, Wilfredo ^{[3, 6]} ; Zuniga, Concepcion ^[7] ; Calzada, Jose ^{[2, 8]} ; Saldana, Azael ^{[2, 9]} ; Corbett, Carlos E. P. ^[3] ; Silveira, Fernando T. ^{[10, 11]} ; Laurenti, Marcia D. ^[3]

Affiliation:Show less -

^[1] Univ Sao Paulo, Fac Med, Dept Patol, Lab Patol Molestias Infecciosas, Av Doutor Arnaldo 455, BR-01246903 Sao Paulo, SP - Brazil

^[2] Inst Conmemorat Gorgas Estudios Salud, Dept Parasitol Mol, Ave Justo Arosemena, Calidonia 081602593 - Panama

^[3] Araujo Flores, Gabriela, V, Univ Sao Paulo, Fac Med, Dept Patol, Lab Patol Molestias Infecciosas, Av Doutor Arnaldo 455, BR-01246903 Sao Paulo, SP - Brazil

^[4] Sao Paulo State Univ Unesp, Inst Biosci, Sao Vicente, SP - Brazil

^[5] Sao Paulo State Univ UNESP, Inst Adv Studies Ocean, Sao Vicente, SP - Brazil

^[6] Univ Nacl Autonoma Honduras, Inst Invest Microbiol, Tegucigalpa - Honduras

^[7] Hosp Escuela Tegucigalpa, Dept Vigilancia Salud, Tegucigalpa - Honduras

^[8] Univ Panama, Fac Med Vet, Campus Harmodio Arias Madrid, Av Juan Pablo II, Albrook - Panama

^[9] Univ Panama, Fac Med, Ctr Invest & Diagnost Enfermedades Parasitarias, Ave Octavio Mendez Pereira, Panama City - Panama

^[10] Inst Evandro Chagas, Dept Parasitol, Belem, PA - Brazil

^[11] Univ Fed Para, Nucleo Med Trop, Belem, PA - Brazil

• Document type: Journal article
• Source: JOURNAL OF IMMUNOLOGY RESEARCH; v. 2021, APR 12 2021.
• Web of Science Citations: 0

Abstract

Macrophages play important roles in the innate and acquired immune responses against Leishmania parasites. Depending on the subset and activation status, macrophages may eliminate intracellular parasites; however, these host cells also can offer a safe environment for Leishmania replication. In this sense, the fate of the parasite may be influenced by the phenotype of the infected macrophage, linked to the subtype of classically activated (M1) or alternatively activated (M2) macrophages. In the present study, M1 and M2 macrophage subsets were analyzed by double-staining immunohistochemistry in skin biopsies from patients with American cutaneous leishmaniasis (ACL) caused by L. (L.) amazonensis, L. (V.) braziliensis, L. (V.) panamensis ,and L. (L.) infantum chagasi. High number of M1 macrophages was detected in nonulcerated cutaneous leishmaniasis (NUCL) caused by L. (L.) infantum chagasi (M1=112 +/- 12, M2=43 +/- 12 cells/mm(2)). On the other side, high density of M2 macrophages was observed in the skin lesions of patients with anergic diffuse cutaneous leishmaniasis (ADCL) (M1=195 +/- 25, M2=616 +/- 114), followed by cases of localized cutaneous leishmaniasis (LCL) caused by L. (L.) amazonensis (M1=97 +/- 24, M2=219 +/- 29), L. (V.) panamensis (M1=71 +/- 14, M2=164 +/- 14), and L. (V.) braziliensis (M1=50 +/- 13, M2=53 +/- 10); however, low density of M2 macrophages was observed in NUCL. The data presented herein show the polarization of macrophages in skin lesions caused by different Leishmania species that may be related with the outcome of the disease. (AU)

• FAPESP's process: 14/50315-0 - Leishmaniasis in Latin America: an advanced perspective on immunopathogenetic factors of cutaneous and visceral infection, immunomodulators of the sandflies vector saliva and immunogenic exo-antigens of Leishmania (L.) infantum chagasi as vaccine candidates
• Grantee: Carlos Eduardo Pereira Corbett
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 17/24834-9 - Evaluation of the experimental infection in hamster by isolated parasites of atypical cutaneous lesion and human visceral caused by Leishmania (L.) infantum chagasi in the municipality of Amapala, Valle, Honduras
• Grantee: Gabriela Venicia Araujo Flores
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/01154-7 - Non-ulcerated or atypical cutaneous leishmaniasis caused by Leishmania infantum chagasi in Amapala municipality, Valle, Honduras: immune-histological characterization of the skin lesions
• Grantee: Carmen Maria Sandoval Pacheco
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 18/04698-6 - Non-ulcerated or atipycal Cutaneous Leishmaniasis caused by Leishmania (L.) infantum chagasi in the municipality of Amapala, Valle, Honduras: immunohistopathological characterization of skin lesions with emphasis in the participation of antigen presenting cells
• Grantee: Carmen Maria Sandoval Pacheco
• Support type: Scholarships in Brazil - Doctorate