Endosymbiont Capture, a Repeated Process of Endosymbiont Transfer with Replacement in Trypanosomatids Angomonas spp.

Author(s):Show less -

Skalicky, Tomas ^[1] ; Alves, Joao M. P. ^[2] ; Morais, Anderson C. ^[2] ; Reznarova, Jana ^[3] ; Butenko, Anzhelika ^{[1, 3]} ; Lukes, Julius ^{[1, 4]} ; Serrano, Myrna G. ^[5] ; Buck, Gregory A. ^[5] ; Teixeira, Marta M. G. ^[2] ; Camargo, Erney P. ^[2] ; Sanders, Mandy ^[6] ; Cotton, James A. ^[6] ; Yurchenko, Vyacheslav ^{[3, 7]} ; Kostygov, Alexei Y. ^{[3, 8]}

Affiliation

^[1] Czech Acad Sci, Biol Ctr, Inst Parasitol, Ceske Budejovice Budweis 37005 - Czech Republic

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, BR-05508000 Sao Paulo - Brazil

^[3] Univ Ostrava, Fac Sci, Life Sci Res Ctr, Ostrava 71000 - Czech Republic

^[4] Univ South Bohemia, Fac Sci, Ceske Budejovice Budweis 37005 - Czech Republic

^[5] Virginia Commonwealth Univ, Dept Microbiol & Immunol, Richmond, VA 23298 - USA

^[6] Wellcome Sanger Inst, Wellcome Genome Campus, Cambridge CB10 1SA - England

^[7] Sechenov Univ, Martsinovsky Inst Med Parasitol, Moscow 119435 - Russia

^[8] Russian Acad Sci, Zool Inst, St Petersburg 199034 - Russia

• Document type: Journal article
• Source: PATHOGENS; v. 10, n. 6 JUN 2021.
• Web of Science Citations: 0

Abstract

Trypanosomatids of the subfamily Strigomonadinae bear permanent intracellular bacterial symbionts acquired by the common ancestor of these flagellates. However, the cospeciation pattern inherent to such relationships was revealed to be broken upon the description of Angomonas ambiguus, which is sister to A. desouzai, but bears an endosymbiont genetically close to that of A. deanei. Based on phylogenetic inferences, it was proposed that the bacterium from A. deanei had been horizontally transferred to A. ambiguus. Here, we sequenced the bacterial genomes from two A. ambiguus isolates, including a new one from Papua New Guinea, and compared them with the published genome of the A. deanei endosymbiont, revealing differences below the interspecific level. Our phylogenetic analyses confirmed that the endosymbionts of A. ambiguus were obtained from A. deanei and, in addition, demonstrated that this occurred more than once. We propose that coinfection of the same blowfly host and the phylogenetic relatedness of the trypanosomatids facilitate such transitions, whereas the drastic difference in the occurrence of the two trypanosomatid species determines the observed direction of this process. This phenomenon is analogous to organelle (mitochondrion/plastid) capture described in multicellular organisms and, thereafter, we name it endosymbiont capture. (AU)

• FAPESP's process: 16/07487-0 - Expanding and barcoding the trypanosomatid culture collection of the University of São Paulo (TCC-USP) and its utilization for phylogenetic and taxonomical studies
• Grantee: Erney Felicio Plessmann de Camargo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/14622-3 - Comparative genomics of Trypanosomatidae
• Grantee: João Marcelo Pereira Alves
• Support Opportunities: Research Grants - Young Investigators Grants