Evaluation of PAMAM Dendrimers (G3, G4, and G5) in the Construction of a SPR-based Immunosensor for Cardiac Troponin T

Author(s)

Giarola, Juliana de F. ^{[1, 2]} ; Souto, Denio E. P. ^[3] ; Kubota, Lauro T. ^{[1, 2]}

Affiliation

^[1] Univ Campinas UNICAMP, Inst Chem, BR-13083970 Campinas, SP - Brazil

^[2] Natl Inst Sci & Technol Bioanalyt INCTBio, Campinas, SP - Brazil

^[3] Fed Univ Parana UFPR, Dept Chem, BR-81530900 Curitiba, Parana - Brazil

• Document type: Journal article
• Source: Analytical Sciences; v. 37, n. 7, p. 1007-1013, JUL 2021.
• Web of Science Citations: 1

Abstract

An immunosensor was developed using a SAM of an alkanethiol associated with PAMAM(G4) dendrimers based on surface plasmon resonance (SPR) to enhance the sensitivity for troponin T detection in blood samples. The feasibility of using three-dimensional platforms based on dendrimers for the development of immunosensors was demonstrated by evaluating three different generations of these dendrimers (G3, G4, and G5) to detect troponin T. The results showed the efficiency of these 3D platforms in anchoring biomolecules, amplifying the detection of troponin T. The sandwich assay showed good performance for troponin T detection, using secondary monoclonal antibodies, in the concentration range of 5 - 300 ng mL(-1) (0.14 - 8.67 nmol L-1), R-2 = 0.991, with the LOD of 3.6 ng mL(-1). The sandwich assay's applicability was demonstrated by evaluating a secondary polyclonal antibody's performance in the concentration range of 3 - 30 ng mL(-1), R-2 = 0.998, with the LOD of 0.98 ng mL(-1). The immunosensor was applied to determine troponin T in blood plasma samples from healthy patients, with an average recovery of 88 to 104%. The performance of the SPR-based immunosensor indicates reliable results and is expected to contribute to the rapid diagnosis of heart attack, with reduced costs. (AU)

• FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
• Grantee: Marco Aurelio Zezzi Arruda
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/08166-3 - Development of an electrochemical immunosensor based on lateral flow for cardiac markers detection
• Grantee: Juliana de Fátima Giarola
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/22127-2 - Development of novel materials strategic for integrated analytical devices
• Grantee: Lauro Tatsuo Kubota
• Support Opportunities: Research Projects - Thematic Grants