Photodynamic therapy with a new bacteriochlorin derivative: Characterization and in vitro studies

Author(s)

Pratavieira, Sebastiao ^[1] ; Uliana, Marciana Pierina ^{[2, 3]} ; dos Santos Lopes, Nahryda Samara ^{[4, 5]} ; Donatoni, Maria Carolina ^[3] ; Linares, Diana Rodriguez ^[2] ; Anibal, Fernanda de Freitas ^{[4, 5]} ; de Oliveira, Kleber Thiago ^[3] ; Kurachi, Cristina ^[1] ; Oliveira de Souza, Clovis Wesley ^{[4, 5]}

Affiliation

^[1] Univ Sao Paulo, Sao Carlos Inst Phys, POB 369, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Fed Integracao Latinoamer, Caixa Postal 2044, BR-85866000 Foz Do Iguacu, PR - Brazil

^[3] Univ Fed Sao Carlos, Dept Quim, Rodovia Washington Luis, Km 235-SP 310, BR-13565905 Sao Carlos, SP - Brazil

^[4] Univ Fed Sao Carlos, Pos Grad Biotecnol, Rodovia Washington Luis, Km 235-SP 310, BR-13565905 Sao Carlos, SP - Brazil

^[5] Univ Fed Sao Carlos, Dept Morfol & Patol, Rodovia Washington Luis, Km 235-SP 310, BR-13565905 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 34, JUN 2021.
• Web of Science Citations: 0

Abstract

Photodynamic therapy presents a therapeutic choice that can be utilized to treat diverse neoplasms. In this technique, the critical element is a photosensitive molecule that absorbs light energy and transfers it to molecular oxygen or biological molecules to form reactive oxygen species, thus inducing irreversible damage to target cells and ultimately leading to cell death. Bacteriochlorin derivatives are employed as photosensitizers (PSs), possessing light-absorbing capacity in the near-infrared region. The objective of this study was to prepare a semisynthetic bacteriochlorin from Rhodopseudomonas faecalis and adding Trizma (R) to improve solubility. Cell viability tests, flow cytometry (apoptotic and necrotic cells were identified by Annexin V and propidium iodide), and confocal microscopy were used to evaluate the photoactivity of bacteriochlorin-Trizma (Bchl-T) in fibroblast (HFF-1-control cells) and breast cancer (MCF-7 cells-target cells) cells. At concentrations above 0.5 mu M, Bchl-T demonstrated 80 % cell death, presenting the highest PS interaction (via fluorescence microscopy) with lysosomes, mitochondria, and the endoplasmic reticulum; the cell death type was revealed as apoptosis (via cytometry). Our findings indicated the suitability of Bchl-T for future application in photodynamic therapy against cancer cells by inducing apoptosis. (AU)

• FAPESP's process: 09/54035-4 - Facility for advanced studies of biosystems and nanostructured materials
• Grantee: Igor Polikarpov
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 14/50857-8 - National Institute in Basic Optics and Applied to Life Sciences
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 08/57858-9 - National Institute for Optics and Photonics
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC