| Full text | |
| Author(s): |
Lemos, Leandro Nascimento
[1]
;
Manoharan, Lokeshwaran
[2, 3]
;
Mendes, Lucas William
[1]
;
Venturini, Andressa Monteiro
[1]
;
Pylro, Victor Satler
[4]
;
Tsai, Siu Mui
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Ctr Nucl Energy Agr CENA, Cell & Mol Biol Lab, Piracicaba - Brazil
[2] Univ Vienna, Dept Ecogen & Syst Biol, Div Archaea Biol & Ecogen, Vienna - Austria
[3] Lund Univ, Natl Bioinformat Infrastruct Sweden NBIS, Lund - Sweden
[4] Fed Univ Lavras UFLA, Dept Biol, Microbial Ecol & Bioinformat Lab, Lavras - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | ENVIRONMENTAL MICROBIOLOGY REPORTS; v. 12, n. 6, p. 651-655, DEC 2020. |
| Web of Science Citations: | 3 |
| Abstract | |
Soil microbiome is one of the most heterogeneous biological systems. State-of-the-art molecular approaches such as those based on single-amplified genomes (SAGs) and metagenome assembled-genomes (MAGs) are now improving our capacity for disentailing soil microbial-mediated processes. Here, we analysed publicly available datasets of soil microbial genomes and MAG's reconstructed from the Amazon's tropical soil (primary forest and pasture) and active layer of permafrost, aiming to evaluate their genome size. Our results suggest that the Candidate Phyla Radiation (CPR)/Patescibacteria phyla have genomes with an average size fourfold smaller than the mean identified in the RefSoil database, which lacks any representative of this phylum. Also, by analysing the potential metabolism of 888 soil microbial genomes, we show that CPR/Patescibacteria representatives share similar functional profiles, but different from other microbial phyla and are frequently neglected in the soil microbial surveys. Finally, we argue that the use of MAGs may be a better choice over SAGs to expand the soil microbial databases, like RefSoil. (AU) | |
| FAPESP's process: | 17/24037-1 - Diversity and evolution of functional genomic traits associated with methane and nitrogen cycles in amazonian soil metagenomes |
| Grantee: | Leandro Nascimento Lemos |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 17/09643-2 - Soil microbial communities in forest-to-pasture conversion: a multi-omics approach |
| Grantee: | Andressa Monteiro Venturini |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 14/50320-4 - Dimensions US-BIOTA - São Paulo: collaborative research: integrating dimensions of microbial biodiversity across land use change in tropical forests |
| Grantee: | Tsai Siu Mui |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |
| FAPESP's process: | 16/18215-1 - Multi-omics integrated and in silico modelling of Archaea communities associated with methanogenis in amazon soils. |
| Grantee: | Leandro Nascimento Lemos |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 15/13546-7 - Microbial community resilience capacity assessed by metagenomics and metatranscriptomics in Amazon forest and pasture soils |
| Grantee: | Andressa Monteiro Venturini |
| Support Opportunities: | Scholarships in Brazil - Doctorate |