Human astrovirus types 1, 4 and 5 circulating among children with acute gastroenteritis in a rural Brazilian state, 2010-2016

Author(s):Show less -

Luchs, Adriana ^[1] ; Tardy, Kaelan ^[2] ; Tahmasebi, Roozbeh ^[2] ; Morillo, Simone Guadagnucci ^[1] ; Milagres, Flavio Augusto de Padua ^{[3, 4]} ; Morais, Vanessa dos Santos ; Brustulin, Rafael ^{[3, 4]} ; Teles, Maria da Aparecida Rodrigues ^[4] ; de Azevedo, Lais Sampaio ^[1] ; de Souza, Ellen Viana ^[1] ; Medeiros, Roberta Salzone ^[1] ; de Souza, Yasmin Franca Viana Pires ^[1] ; Araujo, Emerson Luiz Lima ^[5] ; Witkin, Steven S. ^{[6, 2]} ; Deng, Xutao ^{[7, 8]} ; Delwart, Eric ^{[7, 8]} ; Sabino, Ester Cerdeira ^[2] ; Leal, Elcio ^[9] ; da Costa, Antonio Charlys ^[2]

Affiliation

^[1] Ctr Virol, Inst Adolfo Lutz, Nucleo Doencas Enter, Av Dr Arnaldo S-N 355, BR-01246902 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Med Trop, Dr Eneas Carvalho Aguiar, BR-05403000 Sao Paulo, SP - Brazil

^[3] Univ Fed Tocantins, Tocantins - Brazil

^[4] Lab Cent Saude Publ Tocantins Lacen, Tocantins - Brazil

^[5] Secretaria Vigilancia Saude Minist Saude Brasil, Coordenacao Geral Lab Saude Publ, Dept Articulacao Estrateg Vigilancia Saude, CGLAB DAEVS SVS MS, Brasilia, DF - Brazil

^[6] Weill Cornell Med, Dept Obstet & Gynecol, New York, NY - USA

^[7] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA - USA

^[8] Vitalant Res Inst, San Francisco, CA - USA

^[9] Fed Univ Para, Inst Ciencias Biolog, Para - Brazil

• Document type: Journal article
• Source: ARCHIVES OF VIROLOGY; v. 166, n. 11 AUG 2021.
• Web of Science Citations: 0

Abstract

This study combined conventional epidemiology of human astroviruses. From 2010 to 2016, 232 stool samples from children under 5 years of age were screened using NGS and conventional RT-PCR followed by genetic analysis in order to investigate the genotypic diversity of classical human astrovirus (HAstV) circulating in Tocantins State, Brazil. HAstV was detected in 16 cases (6.9%). Seven specimens (43.7%; 7/16) were positive according RT-PCR and next-generation sequencing (NGS) to investigate the molecular to both NGS and RT-PCR. NGS and RT-PCR individually revealed six (37.5%; 6/16) and three (18.8%; 3/16) additional positive samples, respectively. Sequencing of the HAstV-positive samples revealed HAstV-1a (9/16), HAstV-4c (3/16), and HAstV-5c (4/16) lineages. (AU)

• FAPESP's process: 19/21706-5 - Viral metagenomics of mosquitoes captured in the State of São Paulo
• Grantee: Vanessa dos Santos Morais
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 15/12944-9 - Investigating the evoltution of animal rotavirus strains infecting humans
• Grantee: Adriana Luchs
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/00021-9 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
• Grantee: Antonio Charlys da Costa
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 20/11182-6 - Molecular epidemiology of G8 rotavirus strains isolated in Brazil associated with P[4], P[6] or P[8] genotypes
• Grantee: Roberta Salzone Medeiros
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 20/02469-0 - Full genotype constellation of five rare G12P[9] rotavirus strains detected in Brazil during 2011-2013
• Grantee: Yasmin França Viana Pires de Souza
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 16/01735-2 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
• Grantee: Ester Cerdeira Sabino
• Support Opportunities: Regular Research Grants