Assessment of the Bioactive Potential of Cheese Whey Protein Hydrolysates Using Immobilized Alcalase

Author(s)

da Cruz, Clariana Zanutto Paulino ^[1] ; de Mendonca, Ricardo Jose ^[2] ; Guimaraes, Luis Henrique Souza ^[3] ; dos Santos Ramos, Matheus Aparecido ^[4] ; Garrido, Saulo Santesso ^[1] ; de Paula, Ariela Veloso ^[4] ; Monti, Rubens ^[4] ; Massolini, Gabriella ^[5]

Affiliation

^[1] Sao Paulo State Univ UNESP, Chem Inst, BR-14800060 Araraquara, SP - Brazil

^[2] Fed Univ Triangulo Mineiro UFTM, Biochem Sect, BR-38015050 Uberaba, MG - Brazil

^[3] Sao Paulo Univ USP, Fac Philosophy Sci & Letters Ribeirao Preto, BR-14040901 Sao Paulo, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Fac Pharmaceut Sci, BR-14800903 Araraquara, SP - Brazil

^[5] Univ Pavia, Dipartimento Sci Farm, I-27100 Pavia, PV - Italy

• Document type: Journal article
• Source: Food and Bioprocess Technology; v. 13, n. 12, p. 2120-2130, DEC 2020.
• Web of Science Citations: 0

Abstract

The hydrolysis of bovine cheese whey (BCW) proteins was performed by the Alcalase immobilized on the support glyoxyl-corn-cob-powder (AGCCP). The whey protein hydrolysates (WPH) obtained with AGCPP were fractionated by RP-HPLC according to the hydrophilicity of the peptides (F1, hydrophilic; F2, intermediate hydrophilicity; and F3, hydrophobic). The fractions were analyzed by MALDI-TOF, and molecular weight peptides (< 1500 m/z) were identified. Total WPH obtained by AGCCP presented high capacity reduction of the ABTS radical (57.82%) and high chelating activity of iron II (76.2%). The obtained peptides showed high antimicrobial activity (87.75-100%) against the species Escherichia coli (ATCC 43895) and Listeria monocytogenes (ATCC 7644). Only F2 was effective against the Candida albicans strain ATCC 18804 tested (MIC = 10 mg/mL). The results of this study show that the hydrolysis of BCW proteins using the AGCCP derivative represents a very interesting and viable way for obtention of bioactive peptides from cheese whey. (AU)

• FAPESP's process: 12/07680-4 - Partial hydrolysates of whey proteins from bovine and buffalo milk obtained with immobilised proteases: peptides transportation and absorption through dialyzability and Caco-2 cell culture
• Grantee: Juliana Cristina Bassan
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/12563-2 - Agro industrial residues as solid support for immobilization and stabilization of enzymes of biotechnological interest
• Grantee: Rubens Monti
• Support Opportunities: Regular Research Grants