Assembly of Nano-Biocatalyst for the Tandem Hydrolysis and Reduction of p-Nitrophenol Esters

Author(s)

Barros, Heloise Ribeiro ^{[1, 2]} ; Tanaka, Livia Yukari ^[1] ; Pacheco da Silva, Rafael Trivella ^[1] ; Santiago-Arcos, Javier ^[2] ; Cordoba Torresi, I, Susana ; Lopez-Gallego, Fernando ^{[3, 2]}

Affiliation

^[1] I, Univ Sao Paulo, Dept Fundamental Chem, Inst Chem, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil

^[2] Basque Res & Technol Alliance BRTA, CIC BiomaGUNE, Paseo Miramon 182, San Sebastian 20014 - Spain

^[3] Ikerbasque, Basque Fdn Sci, Bilbao 48013 - Spain

• Document type: Journal article
• Source: PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION; v. 38, n. 10 AUG 2021.
• Web of Science Citations: 0

Abstract

Hybrid nano-biomaterials are exploited in the design and performance of chemo-enzymatic cascades. In this study, lipase is immobilized from Candida antarctica fraction B (CALB) and gold nanoparticles (Au NPs) on magnetic particles coated with silica (MNP@SiO2) to stepwise hydrolyze and reduce p-nitrophenyl esters in tandem reaction. The assembly of the two catalysts at the interface of the MNP@SiO2 particles and the temporal control of the reaction turns out to be the most determinant parameters for the cascade kinetics. When both CALB and Au NPs are co-immobilized at the MNP@SiO2 particle, the tandem reactions take place significantly faster than when both catalysts are physically segregated by their immobilization on different MNP@SiO2 particles. Herein, it is demonstrated that the co-immobilization of biocatalysts and nanocatalysts in solid materials creates hybrid interfaces that accelerated chemo-enzymatic tandem reactions. (AU)

• FAPESP's process: 19/19551-3 - Synthesis of controlled hybrid materials: development of a bionanoplasmonic catalyst in light-driven one-pot cascade reactions
• Grantee: Livia Yukari Tanaka
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• FAPESP's process: 18/02435-8 - Synthesis of controlled hybrid materials based on Fe3O4 for catalytic applications
• Grantee: Livia Yukari Tanaka
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/20892-4 - Nanobioplasmonic catalysts: a novel approach to controlling the selectivity in light-driven one-pot reactions
• Grantee: Heloise Ribeiro de Barros
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/26308-7 - Optimization of the physicochemical properties of nano -structured materials for applications in molecular recognition, catalysis and energy conversion/storage
• Grantee: Roberto Manuel Torresi
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/13492-2 - Synthetic and natural scaffolds applied to regenerative medicine
• Grantee: Luiz Henrique Catalani
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/09668-0 - Understanding the biophysiochemical interactions between plasmonic nanoparticles and enzymes to obtain bio-nanomaterials with controlled properties
• Grantee: Heloise Ribeiro de Barros
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor