A colorimetric microfluidic paper-based analytical device for sulfonamides in cow milk using enzymatic inhibition

Author(s)

Rafanhin Sousa, Ana Carolina ^{[1, 2]} ; Makara, Cleyton Nascimento ^{[1, 2]} ; Brazaca, Lais Canniatti ^{[1, 2]} ; Carrilho, Emanuel ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil

^[2] Inst Nacl Ciencia & Tecnol Bioanalit INCTBio, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: Food Chemistry; v. 356, SEP 15 2021.
• Web of Science Citations: 1

Abstract

To increase milk production, antibiotics are administered to animals to provide weight gain and to prevent or treat diseases. The inappropriate use of these substances can lead to antibiotic resistance and allergic reactions and toxic effects to milk consumers. We describe the development of a simple, fast, portable, and low-cost microfluidic paper-based analytical device (mu PAD) to quantify sulfonamides in milk using the inhibition of the colorimetric reaction between carbonic anhydrase (CA) and 4-nitrophenyl acetate. The main advantages presented by the mu PAD include reproducible batch production, simple application, and precise analysis without previous treatment. The mu PAD displayed good linearity (R-2 >= 0.986) in a wide range of sulfonamides in milk (2.5 to 40.0 mu mol L-1), being selective for the drugs even in a highly complex matrix. We expect that this device allows in situ monitoring of milk quality, reducing the prejudicial conditions associated with high concentrations of sulfonamides in milk. (AU)

• FAPESP's process: 18/19750-3 - Exploring C4D detection for the development of innovative and low-cost microfluidic biosensors
• Grantee: Laís Canniatti Brazaca
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/19413-4 - Agriculture in a POCT: precision agriculture using low-cost microfluidic Paper-based analytical devices for chemical analysis
• Grantee: Emanuel Carrilho
• Support Opportunities: Research Grants - Research Partnership for Technological Innovation - PITE