Baccharin and p-coumaric acid from green propolis mitigate inflammation by modulating the production of cytokines and eicosanoikds

Author(s):Show less -

Ferreira, Juliana C. ^[1] ; Reis, Mouzarllem B. ^[1] ; Coelho, Giovanna D. P. ^[1] ; Gastaldello, Gabriel H. ^[1] ; Peti, Ana Paula F. ^[2] ; Rodrigues, Debora M. ^[2] ; Bastos, Jairo K. ^[2] ; Campo, Vanessa L. ^{[2, 1]} ; Sorgi, Carlos A. ^[3] ; Faccioli, Lucia H. ^[2] ; Gardinassi, Luiz G. ^[4] ; Tefe-Silva, Cristiane ^[1] ; Zoccal, Karina F. ^[1]

Affiliation

^[1] Ctr Univ Barao de Maua CBM, Rua Ramos de Azevedo 423, BR-14090180 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Sao Paulo - Brazil

^[4] Univ Fed Goias, Inst Patol Trop & Saue Publ, Goiania, Go - Brazil

• Document type: Journal article
• Source: Journal of Ethnopharmacology; v. 278, OCT 5 2021.
• Web of Science Citations: 0

Abstract

Ethnopharmacological relevance: Green propolis is produced by Apis mellifera honeybees using Baccharis dracunculifolia D.C. (Asteraceae) as substrate. This Southern Brazilian native plant and green propolis have been used in traditional medicine to treat gastric diseases, inflammation and liver disorders. Aim of the study: Investigate the effects of baccharin (Bac) or p-coumaric acid (pCA) isolated from B. dracunculifolia D.C. (Asteraceae) over the inflammation induced by lipopolysaccharide (LPS) in vivo. Materials and methods: Inflammation was induced by LPS injection into air-pouches in mice, which were subsequently treated with Bac or pCA. Lavage fluid was collected from air pouches for the quantification of cellular influx via microscopy, and quantification of inflammatory mediators via colorimetric methods, ELISA and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results: LPS-induced inflammation increased cellular influx and increased the levels of parameters related to vascular permeability and edema formation, such as nitric oxide (NO) and protein extravasation. Moreover, LPS increased the levels of cytokines and eicosanoids in the air-pouches. Importantly, both Bac and pCA suppressed the infiltration of neutrophils, production of NO and protein extravasation. Notably, the compounds promote differential regulation of cytokine and eicosanoid production. Conclusions: Our results suggest that Bac from green propolis directly affects inflammation by inhibiting the production of cytokines and eicosanoids, while pCA may exert direct, but also indirect effects on inflammation by stimulating the production of regulatory effectors such as interkeukin-10 in vivo. (AU)

• FAPESP's process: 19/21938-3 - Effects of compounds isolated from Baccharis dracunculifolia plant, in air pouch model, on inflammatory mediators production
• Grantee: Juliana Cogo Ferreira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/04138-8 - Attainment of chemical, analytical, biological, pharmacological and technological studies to fill the gaps on the development of Brazilian propolis sector
• Grantee: Jairo Kenupp Bastos
• Support Opportunities: Research Projects - Thematic Grants