| Full text | |
| Author(s): |
Ferreira, Jair Camargo
[1]
;
Amaral, Rafael Silva
[1]
;
Cardoso, Leticia Mara
[1]
;
Marchio, Sophia Panelli
[1]
;
Rodrigues, Marcela Aldrovani
[1]
;
Fonseca Alves, Carlos Eduardo
[2]
;
de Meira, Cezinande
[3]
Total Authors: 7
|
| Affiliation: | [1] Univ Franca, Postgrad Program Anim Sci, Franca, SP - Brazil
[2] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Dept Vet Clin, Botucatu, SP - Brazil
[3] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, Botucatu, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | REPRODUCTION IN DOMESTIC ANIMALS; v. 56, n. 9, p. 1176-1183, SEP 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Ozone (O-3) therapy has been used to improve peripheral tissue oxygenation in humans and domestic animals. The goal of the present study was to characterize histological changes in the endometria of healthy equines following tissue exposure to gas mixtures enriched with different concentrations of O-3. Cycling mares without endometrial degeneration were divided into three groups according to treatment (n = 9 mares/group). The uteri from the O-3, 1/2O(3) and control groups were insufflated for 3 min with gas containing 42, 21 and 0 mu g O-3 ml(-1), respectively. Treatments were performed every three days from D0 to D6. Endometrial samples were collected immediately before the first treatment and 24 hr after the last treatment. The following nine histological parameters were evaluated: (i) the number of endometrial blood vessels, (ii) endometrial vascular degree (EVD), (iii) increase rate of blood vessels, (iv) increase rate of EVD, (v) glandular total area, (vi) glandular lumen area, (vii) intraglandular secretion area, (viii) glandular epithelial height and (ix) luminal epithelial height. In the O-3 group, a positive effect from treatment (p < .01) was detected for all vascular parameters (i, ii, iii and iv), glandular total area, intraglandular secretion area and glandular epithelial height. Compared to the control group, the 1/2O(3) group had greater (p < .01) EVD (84.1 +/- 12%) and a higher increase rate of blood vessels (151.9 +/- 47.1%). Uterine insufflation with low or intermediate concentrations of the O-2-O-3 gas mixture induced endometrial angiogenesis. Morphometry, but not morphology, of the endometrial glands was affected by local O-3 therapy. These findings would be of great significance for the development of new therapies for infertility in mares. (AU) | |
| FAPESP's process: | 19/11680-9 - Ultrastructural study of the equine endometrium exposed to ozone |
| Grantee: | Sophia Panelli Marchió |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 19/11450-3 - Effect of intrauterine ozone therapy on endometrial angiogenesis of non-pregnant mares |
| Grantee: | Letícia Mara Cardoso |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 19/22728-2 - Use of ozone gas as an angiogenic agent in the equine endometrium |
| Grantee: | Jair Camargo Ferreira |
| Support Opportunities: | Regular Research Grants |