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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss)

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Author(s):
Fanali, Lara Zacari [1] ; De Oliveira, Classius [2] ; Sturve, Joachim [3]
Total Authors: 3
Affiliation:
[1] Univ Estadual Paulista UNESP, Grad Program Anim Biol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[2] Univ Estadual Paulista Unesp, Dept Biol, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[3] Univ Gothenburg, Dept Biol & Environm Sci, Box 463, S-40530 Gothenburg - Sweden
Total Affiliations: 3
Document type: Journal article
Source: Environmental Science and Pollution Research; v. 28, n. 38, p. 53926-53935, OCT 2021.
Web of Science Citations: 0
Abstract

Fish have defense systems that are capable of repairing damages caused by xenobiotics like benzo{[}a]pyrene (BaP), so the aims of this study were to identify BaP toxicity in melanomacrophages (MMs) cytoskeleton, evaluate the melanin area in MMs, and analyze genotoxicity. Rainbow trout juveniles (n = 24) were split in 48h and 7d treatments that received 2 mg/kg of BaP. After the experiment, blood samples were collected and liver was removed, to proceed with the analysis: EROD activity, MMs melanin area quantification, melanosomes movements, and a genotoxicity test. The results revealed increased in EROD activity after 48-h and 7-day BaP exposure. The group 7d displayed a reduction in MMs pigmented area, melanosomes aggregation, in addition to an increased frequency of micronucleus. By means of the EROD assay, it was possible to confirm the activation of BaP biotransformation system. The impairment of the melanosomes' movements possibly by an inactivation of the protein responsible for the pigment dispersion consequently affects the melanin area and thus might negatively impact the MMs detoxification capacity. In addition to this cytotoxicity, the increased frequency of micronucleus might also indicate the genotoxicity of BaP in this important fish species. (AU)

FAPESP's process: 18/07989-1 - Effects of benzo[a]pyrene on the cytoskeleton and melanin dispersion of hepatic melanomacrophages of fish and amphibians
Grantee:Lara Zácari Fanali
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 13/02067-5 - Toxic effects of the herbicides Atrazine and Roundup in Eupemphix nattereri (Leiuperidae) and Rhinella schneideri (Bufonidae): morphological changes under different concentrations and abiotic conditions (pH, temperature and photoperiod)
Grantee:Classius de Oliveira
Support Opportunities: Regular Research Grants
FAPESP's process: 18/01078-7 - The liver of anuran amphibians: morphology compared between 10 species and action of benzopyrene in the melanomacrophages of Physalaemus cuvieri
Grantee:Classius de Oliveira
Support Opportunities: Regular Research Grants