| Full text | |
| Author(s): |
Luizon, Marcelo R.
[1]
;
Conceicao, Izabela M. C. A.
[1]
;
Viana-Mattioli, Sarah
[2]
;
Caldeira-Dias, Mayara
[2]
;
Cavalli, Ricardo C.
[3]
;
Sandrim, Valeria C.
[2]
Total Authors: 6
|
| Affiliation: | [1] Univ Fed Minas Gerais, Inst Biol Sci, Dept Genet Ecol & Evolut, Belo Horizonte, MG - Brazil
[2] Univ Estadual Paulista, Inst Biosci, UNESP, Dept Biophys & Pharmacol, Botucatu, SP - Brazil
[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Gynecol & Obstet, Ribeirao Preto, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | FRONTIERS IN PHYSIOLOGY; v. 12, SEP 22 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
MicroRNAs (miRNAs) play an important role in the pathophysiology of preeclampsia (PE). However, the expression of circulating miRNAs was not analyzed in the second trimester of pregnancy, a period of major relevance to identify predictive biomarkers for PE. Therefore, we examined the expression profiles of 84 circulating miRNAs using a PCR array in plasma collected between 20 and 25 weeks of gestation from pregnant women, who subsequently developed PE and those who remained healthy during pregnancy, randomly selected from a prospective cohort. Overall, 23 miRNAs had a fold change > 2.0 and were considered to be upregulated in plasma from pregnant women who subsequently developed PE, even before the onset of clinical symptoms of PE. However, only miR-204-5p was statistically significant (P = 0.0082). Experimentally validated interactions for the target genes of miR-204-5p extracted from miRTarBase were used in the gene set functional enrichment analysis to identify Reactome pathways. The network connecting the 37 target genes for miR-204-5p revealed pathways of known pathophysiological relevance during the early development of PE and included key genes related to PE, such as BDNF, MMP-9, MALAT1, TGFBR2, and SIRT1. We further depicted downstream targets of SIRT1 that are related to the vascular endothelial function or implicated in the pathophysiology of PE, namely, FOXO1, NF kappa B, HIF-1 alpha, NOS3, and PPAR-gamma. Our novel findings provide for circulating miRNAs upregulated in the second trimester on plasma from pregnant women who subsequently developed PE that is potentially related to the early development of PE, which may guide further studies focused on the validation of potential predictive biomarkers in PE.</p> (AU) | |
| FAPESP's process: | 15/20461-8 - Role of NRF2 and HO-1 in preeclampsia: polymorphisms, circulating factors and in vitro assays |
| Grantee: | Valeria Cristina Sandrim |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 08/53593-0 - Etiological factors of preterm birth and consequences of perinatal factors in child health: birth cohorts in two Brazilian cities (BRISA project) |
| Grantee: | Marco Antonio Barbieri |
| Support Opportunities: | Research Projects - Thematic Grants |