| Full text | |
| Author(s): |
Freitas Mariano, Kelli Cristina
[1]
;
Boava Papini, Juliana Zampoli
[2]
;
de Faria, Naially Cardoso
[3]
;
Cabral Heluany, Daniele Nicoli
[2]
;
Lourenconi Botega, Ana Luiza
[2]
;
Saia Cereda, Cintia Maria
[2]
;
de Paula, Eneida
[4]
;
Tofoli, Giovana Radomille
[2]
;
de Araujo, Daniele Ribeiro
[1, 5]
Total Authors: 9
|
| Affiliation: | [1] Fed Univ ABC, Human & Nat Sci Ctr, Santo Andre, SP - Brazil
[2] Sao Leopoldo Mandic Res Inst, Sao Leopoldo Mandic Fac, Campinas, SP - Brazil
[3] Univ Fed Sao Paulo, Translat Med Postgrad Program, Sao Paulo - Brazil
[4] Univ Estadual Campinas, Dept Biochem, Campinas, SP - Brazil
[5] Fed Univ ABC, Drugs & Bioact Delivery Syst Res Grp SISLIBIO, Av Estados, 5001 Bl A, T3, Lab 503-3 Bangu, Santo Andre, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | BIOMED RESEARCH INTERNATIONAL; v. 2021, SEP 16 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
This study reports the development of thermosensitive hydrogels for delivering ropivacaine (RVC), a wide clinically used local anesthetic. For this purpose, poloxamer- (PL-) based hydrogels were synthesized for evaluating the influence of polymer concentration, hydrophilic-lipophilic balances, and binary system formation on biopharmaceutical properties and pharmacological performance. Transition temperatures were shifted, and rheological analysis revealed a viscoelastic behavior with enhanced elastic/viscous modulus relationship (G/G `' = 1.8 to 22 times), according to hydrogel composition and RVC incorporation. The RVC release from PL407 and PL407/338 systems followed the Higuchi model (R-2 = 0.923-0.989), indicating the drug diffusion from hydrogels to the medium. RVGPL hydrogels were potentially biocompatible evoking low cytotoxic effects (in fibroblasts and Schwann cells) and mild/moderate inflammation signs on sciatic nerve nearby histological evaluation. In vivo pharmacological assays demonstrated that PL407 and PL407/338 evoked differential analgesic effects, by prolonging the sensory blockade duration up to similar to 340 and 250 min., respectively. All those results highlighted PL407 and PL407/338 as promising new strategies for sustaining analgesic effects during the postoperative period. (AU) | |
| FAPESP's process: | 14/14457-5 - Lipid-based nanocarriers (SLN/NLC and remote-loading liposomes) used to improve the upload and potency of local anesthetics |
| Grantee: | Eneida de Paula |
| Support Opportunities: | Research Projects - Thematic Grants |