Understanding the differences in 2G ethanol fermentative scales through omics data integration

Author(s):Show less -

Carvalho, Lucas M. ^{[1, 2]} ; Carvalho-Netto, Osmar V. ^[1] ; Calderon, Luige L. ^[1] ; Gutierrez, Milena ^[1] ; De Assis, Michelle A. ^[1] ; Mofatto, Luciana S. ^[1] ; Camargo, Antonio P. ^[1] ; Dos Santos, Leandro V. ^{[1, 3]} ; Borelli, Guilherme ^[1] ; Temer, Beatriz ^[1] ; Araujo, Guido ^{[4, 2]} ; Pereira, Goncalo A. G. ^[1] ; Carazzolle, Marcelo F. ^{[1, 2]}

Affiliation

^[1] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut Microbiol & Immunol, BR-13083862 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Ctr Comp Engn & Sci, BR-13083861 Campinas, SP - Brazil

^[3] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biorenewable Natl Lab LNBR, St Giuseppe Maximo Scolfaro, 10000 Bosque Palmeira, BR-13083100 Campinas, SP - Brazil

^[4] Univ Estadual Campinas, Inst Comp, BR-13083852 Campinas, SP - Brazil

• Document type: Journal article
• Source: FEMS Yeast Research; v. 21, n. 4 JUN 2021.
• Web of Science Citations: 0

Abstract

In this work, we evaluated the fermentative performance and metabolism modifications of a second generation (2G) industrial yeast by comparing an industrial condition during laboratory and industrial scale fermentations. Fermentations were done using industrial lignocellulosic hydrolysate and a synthetic medium containing inhibitors and analyses were carried out through transcriptomics and proteomics of these experimental conditions. We found that fermentation profiles were very similar, but there was an increase in xylose consumption rate during fermentations using synthetic medium when compared to lignocellulosic hydrolysate, likely due to the presence of unknown growth inhibitors contained in the hydrolysate. We also evaluated the bacterial community composition of the industrial fermentation setting and found that the presence of homofermentative and heterofermentative bacteria did not significantly change the performance of yeast fermentation. In parallel, temporal differentially expressed genes (tDEG) showed differences in gene expression profiles between compared conditions, including heat shocks and the presence of up-regulated genes from the TCA cycle during anaerobic xylose fermentation. Thus, we indicate HMF as a possible electron acceptor in this rapid respiratory process performed by yeast, in addition to demonstrating the importance of culture medium for the performance of yeast within industrial fermentation processes, highlighting the uniquenesses according to scales. (AU)

• FAPESP's process: 13/08293-7 - CCES - Center for Computational Engineering and Sciences
• Grantee: Munir Salomao Skaf
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 20/06563-0 - Comparison of fermentative profiles of ethanol fermenting yeasts genetically modified on an industrial and laboratory scale through networks co-expression
• Grantee: Milena Antunes Piccart Gutierrez
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 19/12914-3 - Integrated multi-omics analysis and metabolic network simulations applied to Saccharomyces cerevisiae for second generation ethanol production
• Grantee: Lucas Miguel de Carvalho
• Support Opportunities: Scholarships in Brazil - Post-Doctoral