Screening paracoccidioidomycosis by double immunodiffusion test in a referred diagnostic center in Brazilian southeastern: an accessible tool

Author(s):Show less -

Maifrede, Simone Bravim ^[1] ; Kruschewsky, Wdson Luis Lima ^[2] ; Patricio, Suzana Alves ^[3] ; Falqueto, Aloisio ^[2] ; Pecanha, Paulo Mendes ^[2] ; Malaquias, Luiz Cosme Cotta ^[4] ; Possa, Ana Paula ^[5] ; de Camargo, Zoilo Pires ^[5] ; Rodrigues, Anderson Messias ^[5] ; Goncalves, Sarah Santos ^[1] ; Grao-Velloso, Tania Regina ^[3]

Affiliation

^[1] Fed Univ Espirito Santo UFES, Dept Pathol, Ctr Res Med Mycol, Vitoria - Brazil

^[2] Fed Univ Espirito Santo UFES, Dept Clin Med, Vitoria - Brazil

^[3] Fed Univ Espirito Santo UFES, Dept Dent, 1468 Marechal Campos Ave, BR-29040090 Vitoria, ES - Brazil

^[4] Fed Univ Alfenas UNIFAL MG, Dept Immunol, Alfenas - Brazil

^[5] Fed Univ Sao Paulo Unifesp, Dept Microbiol Immunol & Parasitol, Lab Emerging Fungal Pathogens, Sao Paulo - Brazil

• Document type: Journal article
• Source: INFECTION; v. 49, n. 6 SEP 2021.
• Web of Science Citations: 0

Abstract

Purpose This article shows reports of the clinical-epidemiological characteristics and serological screening in patients assisted by a reference center for PCM care, University Hospital Cassiano Antonio Moraes, Federal University of Espirito Santo, Brazil. Methods The patient's sera with PCM were analyzed by DID test at the beginning and the end treatment. Clinical and demographic data were also collected to characterize the sample. Results One hundred patients with a suspected diagnosis of PCM were evaluated. Serology by DID test was used as a screen in all patients. The test was positive for 79 patients (72 for Paracoccidioides brasiliensis and 7 for Paracoccidioides lutzii). Serology was negative in 21 sera, although all of them were diagnosed PCM by histopathologic or direct exam. Serological follow-up was performed during the treatment of all patients. After treatment, 58(58%) had negative serology and 33(33%) low levels of antibodies (<= 1:16). Conclusion Our results indicate the importance of the DID test for the screening and monitoring of PCM and that the incidence of P. lutzii might be greater than expected in areas where it is not the predominant PCM species. Therefore, this article may contribute to improving the knowledge and clinical management about this disease. (AU)

• FAPESP's process: 18/21460-3 - Study of different antigenic preparations of Paracoccidioides lutzii for the standardization of the ELISA test as an aid in the diagnosis of paracoccidioidomycosis due to P. lutzii
• Grantee: Zoilo Pires de Camargo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/27265-5 - Molecular epidemiology and genomic perspectives on the evolution and spread of emerging fungal pathogens
• Grantee: Anderson Messias Rodrigues
• Support Opportunities: Research Grants - Young Investigators Grants