The impact of Zika virus exposure on the placental proteomic profile

Author(s):Show less -

Rosa-Fernandes, Livia ^{[1, 2]} ; Bandeira, Carla ^[3] ; Pour, Shahab Zaki ^[4] ; Benedetti, Viviane de Fatima ^[4] ; Ferreira, Daniel ^[4] ; Lorenzon, Aline R. ^{[5, 3]} ; Moreli, Jusciele B. ^{[6, 3]} ; Marinho, Claudio Romero Farias ^[2] ; Larsen, Martin R. ^[7] ; Zanotto, Paolo ^[4] ; Palmisano, Giuseppe ^[1] ; Bevilacqua, Estela ^[3]

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, GlycoProte Lab, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Lab Expt Immunoparasitol, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Lab Studies Maternal Fetal Interact & Placenta, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo, SP - Brazil

^[5] Huntington Med Reprod, Sao Paulo, SP - Brazil

^[6] Faceres Sch Med, Sao Jose Do Rio Preto, SP - Brazil

^[7] Univ Southern Denmark, Biochem & Mol Biol Dept, Odense - Denmark

• Document type: Journal article
• Source: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE; v. 1868, n. 1 JAN 1 2022.
• Web of Science Citations: 0

Abstract

Zika virus (ZIKV) infection has caused severe unexpected clinical outcomes in neonates and adults during the recent outbreak in Latin America, particularly in Brazil. Congenital malformations associated with ZIKV have been frequently reported; nevertheless, the mechanism of vertical transmission and the involvement of placental cells remains unclear. In this study, we applied quantitative proteomics analysis in a floating explant model of chorionic villi of human placental tissues incubated with ZIKV and with ZIKV pre-adsorbed with anti-ZIKV envelope protein. Proteomic data are available via ProteomeXchange with PXD025764 identifier Altered levels of proteins were involved in cell proliferation, apoptosis, inflammatory processes, and the integrin-cytoskeleton complex. Antibody-opsonized ZIKV particles differentially modulated the pattern of protein expression in placental cells; this phenomenon may play a pivotal role in determining the course of infection and the role of mixed infections. The expression of specific proteins was also evaluated by immunoperoxidase assays. These data fill gaps in our understanding of early events after ZIKV placental exposure and help identify infection control targets. (AU)

• FAPESP's process: 20/04923-0 - SARS-CoV-2 glycosylation: a blueprint structural insight for understanding COVID-19 pathogenesis
• Grantee: Giuseppe Palmisano
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/18257-1 - Multi-user equipment approved in grant 14/06863-3: HPLC system configured for analysis of carbohydrates, amino acidis, peptides and glycoproteins
• Grantee: Giuseppe Palmisano
• Support Opportunities: Multi-user Equipment Program