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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Plasma membrane permeabilization to explain erythrosine B phototoxicity on in vitro breast cancer cell models

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Author(s):
Bistaffa, Maria J. [1] ; Camacho, Sabrina A. [2, 1] ; Melo, Carlos F. O. R. [1] ; Catharino, Rodrigo R. [3] ; Toledo, Karina A. [1, 4] ; Aoki, Pedro H. B. [1]
Total Authors: 6
Affiliation:
[1] Sao Paulo State Univ, UNESP, Sch Sci Humanities & Languages, BR-19806900 Assis, SP - Brazil
[2] Univ Sao Paulo, Sao Carlos Inst Phys, IFSC, BR-13566590 Sao Carlos, SP - Brazil
[3] Univ Estadual Campinas, Sch Pharmaceut Sci, INNOVARE Biomarkers Lab, BR-13083970 Campinas, SP - Brazil
[4] Sao Paulo State Univ, UNESP, Inst Biosci Letters & Exact Sci, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 223, OCT 2021.
Web of Science Citations: 0
Abstract

Lipid oxidation is ubiquitous in cell life under oxygen and essential for photodynamic therapy (PDT) of carcinomas. However, the mechanisms underlying lipid oxidation in rather complex systems such as plasma membranes remain elusive. Herein, Langmuir monolayers were assembled with the lipid extract of glandular breast cancer (MCF7) cells and used to probe the molecular interactions allowing adsorption of the photosensitizer (PS) erythrosine B and subsequent photooxidation outcomes. Surface pressure (pi) versus area (cm2/mL) isotherms of MCF7 lipid extract shifted to larger areas upon erythrosine incorporation, driven by secondary interactions that affected the orientation of the carbonyl groups and lipid chain organization. Light-irradiation increased the surface area of the MCF7 lipid extract monolayer containing erythrosine owing to the lipid hydroperoxidation, which may further undergo decomposition, resulting in the chain cleavage of phospholipids and membrane permeabilization. Incorporation of erythrosine by MCF7 cells induced slight toxic effects on in vitro assays, differently of the severe phototoxicity caused by light-irradiation, which significantly decreased cell viability by more than 75% at 2.5 x 10-6 mol/L of erythrosine incubated for 3 and 24 h, reaching nearly 90% at 48 h of incubation. The origin of the phototoxic effects is in the rupture of the plasma membrane shown by the frontal (FSC) and side (SSC) light scattering of flow cytometry. Consistent with hydroperoxide decomposition, membrane permeabilization was also confirmed by cleaved lipids detected in mass spectrometry and subsidizes the necrotic pathway of cell death. (AU)

FAPESP's process: 18/14692-5 - Photothermal therapy mediated by gold nanoparticles: from model systems of biomembranes toward in vitro culture of tumor cells
Grantee:Sabrina Aléssio Camacho
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
Grantee:Osvaldo Novais de Oliveira Junior
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 16/20576-2 - Photosensitization of oropharyngeal (HEp-2) cancer cells induced by the action of the xanthene erythrosin
Grantee:Maria Julia Bistaffa
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 18/16713-0 - Photodynamic therapy: from molecular effects in model systems toward photodynamic efficiency in cell culture
Grantee:Pedro Henrique Benites Aoki
Support Opportunities: Regular Research Grants