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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Brazilian octocoral Phyllogorgia dilatata as a source of cytotoxic compounds

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Author(s):
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THAYSSA S.F. FAGUNDES [1] ; ARTHUR L. MACEDO ; DHIEGO B. RIGATO [3] ; BRUNO S. DO AMARAL ; PAULA CHRISTINE JIMENEZ [5] ; LETÍCIA V. COSTA-LOTUFO [6] ; RENATA F.A. PEREIRA [7] ; FÁBIO AGUIAR-ALVES [8] ; ANGÉLICA R. SOARES [9] ; THATYANA R.A. VASCONCELOS [10] ; QUEZIA B. CASS [11] ; ALESSANDRA L. VALVERDE [12]
Total Authors: 12
Affiliation:
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[1] Universidade Federal Fluminense. Programa de Pós-Graduação em Química - Brasil
[3] Universidade Federal de São Paulo. Instituto do Mar - Brasil
[5] Universidade Federal de São Carlos. Departamento de Química - Brasil
[6] Universidade de São Paulo. Instituto de Ciências Biomédicas - Brasil
[7] Universidade Federal Fluminense. Programa de Pós-Graduação em Microbiologia e Parasitologia Aplicadas - Brasil
[8] Universidade Federal Fluminense. Programa de Pós-Graduação em Microbiologia e Parasitologia Aplicadas - Brasil
[9] Universidade Federal do Rio de Janeiro. Instituto de Biodiversidade e Sustentabilidade - Brasil
[10] Universidade Federal Fluminense. Programa de Pós-Graduação em Química - Brasil
[11] Universidade Federal de São Carlos. Departamento de Química - Brasil
[12] Universidade Federal Fluminense. Programa de Pós-Graduação em Química - Brasil
Total Affiliations: 12
Document type: Journal article
Source: Anais da Academia Brasileira de Ciências; v. 93, n. 4 2021-10-22.
Abstract

Abstract The extensive marine biodiversity has proved to be a promising source of substances with biomedical potential. In this study, the cytotoxicity of the Brazilian octocoral Phyllogorgia dilatata (Gorgoniidae) was evaluated against two tumor cell lines and three bacterial strains. The methanol/dichloromethane crude extract presented no antibacterial activity up to the highest concentration tested (512 µg/mL), however it revealed a noteworthy antiproliferative effect against HCT-116 (80%) and MCF-7 (54%) cell lines at 50 μg/mL. Therefore, guided by the cytotoxic activity, a multistep chemical fractionation of the extract provided the subfraction 5 (PDPH2-5) with IC50 values of 3.18 and 17.80 μg/mL against HCT-116 and MCF-7, respectively. The LC-HRMS/MS analysis of PDPH2-5 showed ions of m/z 219.1742 and 219.1743, characterized as (E,E) and (Z,E) germacrone, after a LC-DAD-SPE/NMR analysis of the hexanic fraction and comparisons of NMR data with the literature. Previously reported assessments to the cytotoxic activity of the (E,E)-diastereoisomer disclosed higher IC50 values than that obtained for the PDPH2-5 fraction, suggesting, herein, a potentiated effect of the diastereoisomeric mixture. Such remark encourage further bioactivity studies with stereoisomer mixtures and reduce the urge for compound isolation. (AU)