Isolation, characterization, antileishmanial and antitrypanosomal activity of bioactive compounds from Brazilian amphibian poisons

• Grant number: 09/12236-3
• Support type: Scholarships in Brazil - Master
• Effective date (Start): March 01, 2010
• Effective date (End): February 29, 2012
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Principal Investigator: André Gustavo Tempone Cardoso
• Grantee: Érika Gracielle Pinto
• Home Institution: Instituto Adolfo Lutz (IAL). Coordenadoria de Controle de Doenças (CCD). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Among the parasitic infectious diseases, those caused by protozoan parasites are a significant challenge for Public Health and are represented by Leishmaniasis and Chagas disease. It affects mainly the poorest population and thus is not recognized as a potential market. The goal of the present work is to isolate novel natural compounds with antileishmanial and antitrypanosomal activities. In this study it will be fractionated using bioguided assays the poison of the amphibians Corythomantis greeningi and Siphonops annulatus through different chromatographic techniques with the focus on active peptides and secondary metabolites. It will also be evaluate the in vitro effectiveness of the isolated compounds against different parasitic life stages of both parasites. Through spectroscopic techniques, as nuclear magnetic resonance and spectrometric techniques as mass spectrometry the chemical structure of the bioactive compounds will be elucidated. By using cytotoxicity assays against mammalian cells it will be evaluated the in vitro Selectivity Index. This study aims to contribute with novel drug leads from natural sources that could be promptly used for drug design and molecular modeling studies for Leishmaniasis and Chagas disease.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PINTO, ERIKA GRACIELLE; ANTONIAZZI, MARTA MARIA; JARED, CARLOS; TEMPONE, ANDRE GUSTAVO. Antileishmanial and antitrypanosomal activity of the cutaneous secretion of Siphonops annulatus. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 20, NOV 24 2014. Web of Science Citations: 5.

PINTO, ERIKA GRACIELLE; PIMENTA, DANIEL C.; ANTONIAZZI, MARTA MARIA; JARED, CARLOS; TEMPONE, ANDRE GUSTAVO. Antimicrobial peptides isolated from Phyllomedusa nordestina (Amphibia) alter the permeability of plasma membrane of Leishmania and Trypanosoma cruzi. Experimental Parasitology, v. 135, n. 4, p. 655-660, DEC 2013. Web of Science Citations: 22.