Investigation of a modified hydraulic calcium silicate-based material – Bio-C Pulpo

Author(s)

Lauter Eston PELEPENKO ^[1] ; Flávia SAAVEDRA ^[2] ; Thiago Bessa Marconato ANTUNES ^[3] ; Gabriela Fernanda BOMBARDA ^[4] ; Brenda Paula Figueiredo de Almeida GOMES ^[5] ; Alexandre Augusto ZAIA ^[6] ; Marina Angélica MARCIANO ^[7]

Affiliation

^[1] Universidade Estadual de Campinas. Piracicaba Dental School. Department of Restorative Dentistry - Brasil

^[2] Universidade Estadual de Campinas. Piracicaba Dental School. Department of Restorative Dentistry - Brasil

^[3] Universidade Estadual de Campinas. Piracicaba Dental School. Department of Restorative Dentistry - Brasil

^[4] Universidade Estadual de Campinas. Piracicaba Dental School. Department of Restorative Dentistry - Brasil

^[5] Universidade Estadual de Campinas. Piracicaba Dental School. Department of Restorative Dentistry - Brasil

^[6] Universidade Estadual de Campinas. Piracicaba Dental School. Department of Restorative Dentistry - Brasil

^[7] Universidade Estadual de Campinas. Piracicaba Dental School. Department of Restorative Dentistry - Brasil

• Document type: Journal article
• Source: Brazilian Oral Research; v. 35, 2021-06-16.

Abstract

Abstract This study evaluated the physicochemical, biological, and antimicrobial properties of a new hydraulic calcium silicate-based modified material, and compared it with MTA Repair HP and MTA Angelus. The materials were assessed regarding color luminosity (L), color change, radiopacity, setting time, and ISO 6876:2012 linear flow. Volumetric filling and volume change were evaluated using microcomputed-tomography (µCT). Chemical characterization after 28 days in Hank’s Balanced Salt Solution (HBSS) and pH analysis were also assessed. Biological characterization of cytotoxicity and microbiological assessment were also undertaken. Shapiro-Wilk, ANOVA, Levene and post hoc analyses with Bonferroni correction were performed, adopting a 5% significance level (p <0.05). Bio-C Pulpo exhibited the highest L values after 90 days. All tested materials demonstrated color change during the analyses, and had radiopacity above 5 mm Al. MTA Repair HP set faster than Bio-C Pulpo, whereas the latter had the highest linear flow. MTA Repair HP had the highest volumetric filling in µCT analysis. Bio-C Pulpo showed the highest alkalinity during all tested periods, and the highest volumetric loss (above 9%), in comparison with MTA Repair HP and MTA Angelus. Bio-C Pulpo did not form calcium hydroxide after hydration. MTA Repair HP demonstrated the highest cytocompatibility, and Bio-C Pulpo, the highest cytotoxicity. No inhibition halos were observed for any material, and similar higher turbidity values were seen after direct contact. Composition additives used in Bio-C Pulpo modified its properties, and both the absence of calcium hydroxide deposition after hydration, and the related cytotoxicity of this material are of particular concern. (AU)

• FAPESP's process: 18/06515-6 - Chemical and structural characterization of high-plasticity reparative cements
• Grantee: Gabriela Fernanda Bombarda
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/16722-9 - Diffraction patterns of high-plasticity reparative cements
• Grantee: Gabriela Fernanda Bombarda
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• FAPESP's process: 17/18413-0 - Evaluation of bioactivity, flowability, pH and calcium release of high-plasticity repair cements
• Grantee: Thiago Bessa Marconato Antunes
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/05096-7 - Evaluation of physical, chemical and antibacterial of a novel high-plasticity repair cement
• Grantee: Marina Angélica Marciano da Silva
• Support Opportunities: Regular Research Grants