Probeless and label-free impedimetric biosensing of D-dimer using gold nanoparticles conjugated with dihexadecylphosphate on screen-printed carbon electrodes

Author(s)

Tasic, Nikola ^[1] ; Cavalcante, Leticia ^[1] ; Deffune, Elenice ^[2] ; Goes, Marcio Sousa ^[3] ; Paixao, Thiago R. L. C. ^[1] ; Goncalves, Luis Moreira ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Sao Paulo, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Botucatu Med Sch, Cell Engn Lab, Blood Transfus Ctr, Botucatu, SP - Brazil

^[3] Univ Fed Integracao Latinoamer UNILA, Inst Latino Amer Ciencias Vida & Nat, Ctr Interdisciplinar Ciencias Nat, Foz Do Iguacu, PR - Brazil

• Document type: Journal article
• Source: Electrochimica Acta; v. 397, NOV 20 2021.
• Web of Science Citations: 0

Abstract

D-dimer (DD) is a clinical biomarker of emerging significance. Its fast analysis and quantification, preferably by the bedside, helps a medical doctor make crucial decisions. Electrochemical biosensors are point-of-care technologies that can address such issues. Herein, it is developed a probeless and label-free impedimetric DD biosensor. Gold nanoparticles (AuNPs) are dispersed along with dihexadecylphosphate (DHP) on the surface of screen-printed carbon electrodes (SPCEs) to attach anti-DD monoclonal antibody as sensing recognition element, and the measurements are performed using electrochemical impedance spectroscopy (EIS). Two different analytical models are used to interpret raw impedance spectra. The first model is based on the capacitive response within the DHP monolayer and its electrochemical occupancy described by the complex capacitance value at the frequency of 200 mHz, noted as Ml. The second model is based on the charge transfer resistance changes (Rct) occurring upon the mAb DD binding event, fitted by two different Randles types of the equivalent circuits (noted as M2a and M2b). The models are compared, and with the obtained high linearity in the clinically relevant range (up to 500 ng mL(-1)) and low levels of detection (as low as 8.92 ng mL(-1)), a potential POC sensor is demonstrated. Additionally, the proposed sensor is applied in real whole blood samples showing good recovery values with certain discrepancies from the standard laboratory assay result, making a cheap and straightforward way for semi-quantitative bedside clinical evaluation. (C) 2021 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 18/13922-7 - Development of a point-of-care biosensor for the swift determination of D-Dimers
• Grantee: Nikola Tasic
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/08782-1 - Miniaturized and integrated chemical sensors: new fabrication platforms for biological, clinical and environmental applications
• Grantee: Mauro Bertotti
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/10522-5 - Development of alternative methods for fabrication of electrochemical paper-based sensors
• Grantee: Thiago Regis Longo Cesar da Paixão
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/14425-7 - A new synergetic path for gas-diffusion microextraction (GDME) making use of molecular imprinted polymers (MIPs)
• Grantee: Luís Francisco Moreira Gonçalves
• Support Opportunities: Research Grants - Young Investigators Grants