Continuous Synthesis of Biodiesel from Outstanding Kernel Oil in a Packed Bed Reactor Using Burkholderia cepacia Lipase Immobilized on Magnetic Nanosupport

Author(s)

Bento, Heitor B. S. ; Reis, Cristiano E. R. ; Pinto, Pedro A. ; Cortez, Daniela V. ; Vilas Boas, Renata N. ; Costa-Silva, Tales A. ; Carvalho, Ana K. F. ; de Castro, Heizir F.

• Document type: Journal article
• Source: CATALYSIS LETTERS; OCT 2021.
• Web of Science Citations: 0

Abstract

This study deals with the use of commercial lipase stabilized onto magnetic particles of iron oxides (Fe3O4/gamma-Fe2O3) and applied as a heterogeneous biocatalyst in the synthesis of ethyl esters (biodiesel) in a solvent-free medium on a continuous process. Magnetic particles were synthesized by alkaline co-precipitation, silanized, activated and subsequently used for immobilization of Burkholderia cepacia lipase. The results regarding the lipase immobilization showed an enzyme activity retention of about 53% and an increase in the K-M value in about threefold (from 410 to 1262 mmol L-1) and a decrease in the V-max value in sevenfold (From 12,390 to 1786 U g(-1)) when compared with the free enzyme. The immobilization process favored the thermal stability and increased the half-time of the enzyme about tenfold at 50 degrees C. The immobilized derivative was evaluated to aroma production and the activity of esterification was calculated as being 56.7 mu mol L-1 g(-1) min(-1), which corresponds to a productivity value of 0.58 g ester L-1 h(-1). The immobilized system was also used to mediate transesterification of kernel oil in a fixed bed reactor operating in a continuous flow with a reaction medium composed of oil and ethanol at a molar ratio of 1:12, 50 degrees C and space-time of 16 h. The operational stability of the immobilized lipase estimated at 47 days allowed to operate the reactor with high productivity of 38.7 +/- 0.7 mg g(-1) h(-1). Also, the product properties of the ester content (> 96.5%) and kinematic viscosity value (5.32 +/- 0.4 mm(2) s(-1) at 40 degrees C) meet the requirements of the ANP and ASTM (D6751) for biodiesel fuel. {[}GRAPHICS] . (AU)

• FAPESP's process: 20/15513-7 - Scale up of L-asparaginase production employing genetically modified microorganism
• Grantee: Heitor Buzetti Simões Bento
• Support Opportunities: Scholarships in Brazil - Post-Doctoral