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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Study of the interactions of gold nanoparticles functionalized with aminolevulinic acid in membrane models

Full text
Author(s):
da Silva, Rafael Leonardo C. G. [1] ; Goncalves, Karina de Oliveira [2] ; Courrol, Lilia Coronato [2] ; Caseli, Luciano [1]
Total Authors: 4
Affiliation:
[1] Univ Fed Sao Paulo, Dept Chem, Diadema, SP - Brazil
[2] Fed Univ Sao Paulo Diadema, Dept Phys, Diadema, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 205, SEP 2021.
Web of Science Citations: 0
Abstract

Gold nanoparticles have been intensively studied in cancer therapy to improve drug release, increasing thera-peutic action and reducing adverse effects. The interaction between gold nanoparticles and cell membranes can give information about the cell internalization. In this study, gold nanoparticles with aminolevulinic acid (5-ALA) were synthesized using the photoreduction method (5-ALA: AuNPs). The prodrug 5-ALA is responsible for protoporphyrin IX synthesis inside the cell and allows the use of therapies as photodynamic and sonodynamic therapies. The cytotoxicity test was performed on a breast cancer tumor line (MCF-7), and high Content Screening assay was applied to evaluate the entry of nanoparticles into cells. DPPS Langmuir monolayers were assembled at the air/water interface and employed as a simplified membrane model for half of a tumorigenic cell membrane. We assessed the molecular interactions between 5-ALA: AuNPs and phospholipids using tensiometry (pi-A isotherms) and vibrational spectroscopy (PM-IRRAS) experiments. We found that the functionalized gold nanoparticles strongly interact with DPPS polar head groups (especially phosphate and carbonyl), changing the phospholipid hydration and leading to a general decrease in the monolayer conformational order. This work then probes that specific interaction between 5-ALA: AuNPs and the negatively charged phospholipid can be assessed using Langmuir monolayers as simplified biomembrane models. (AU)

FAPESP's process: 19/03239-0 - Nanostructured interfaces for the investigation of bioactive substances in cell membrane models and for the construction of optoelectronic devices
Grantee:Luciano Caseli
Support Opportunities: Regular Research Grants
FAPESP's process: 18/00291-9 - Langmuir and Langmuir-Blodgett films of lipids containing phenylalanine dehydrogenase for sensors of phenylalanine
Grantee:Rafael Leonardo Cruz Gomes da Silva
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 17/23686-6 - Use of fluorescence spectroscopy on detection and control of contamination in food
Grantee:Lilia Coronato Courrol
Support Opportunities: Regular Research Grants
FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
Grantee:Osvaldo Novais de Oliveira Junior
Support Opportunities: Research Projects - Thematic Grants