Supercritical CO2 assisted process for the production of mixed phospholipid nanoliposomes: Unloaded and vitamin D-3-loaded vesicles

Author(s)

Chaves, Matheus A. ^{[1, 2]} ; Baldino, Lucia ^[2] ; Pinho, Samantha C. ^[1] ; Reverchon, Ernesto ^[2]

Affiliation

^[1] Univ Sao Paulo, Sch Anim Sci & Food Engn, Dept Food Engn, Lab Encapsulat & Funct Foods LEnAlis, Av Duque de Caxias Norte 225, Pirassununga, SP - Brazil

^[2] Univ Salerno, Dept Ind Engn, Via Giovanni Paolo II, 132, I-84084 Fisciano, SA - Italy

• Document type: Journal article
• Source: Journal of Food Engineering; v. 316, MAR 2022.
• Web of Science Citations: 0

Abstract

In this study, SuperLip, an innovative technology assisted by supercritical carbon dioxide (SC-CO2), was used to produce unloaded and vitamin D-3 (VD3)-loaded nanoliposomes. Vesicles were produced using hydrogenated and nonhydrogenated phosphatidylcholine from food-grade lecithins at ratios of 30:70, 20:80 and 0:100. SuperLip was operated at 100 bar and 40 degrees C using water flow rates ranging from 2.5 to 10 mL/min. The results showed that unloaded liposomes produced by SuperLip presented a unimodal size distribution at a water flow rate of 10 mL/min, regardless of the phospholipid ratio, and mean diameters ranging from 125 to 141 nm. VD3-loaded liposomes also presented a unimodal size distribution at this water flow rate, but slightly higher diameters that ranged from 144 to 252 nm. Furthermore, the addition of 20% purified phospholipids to liposomes led to an increase in the mean size of VD3-loaded vesicles from 144 to 218 nm and an increase in the encapsulation efficiency from 66.7 to 88.9%. (AU)

• FAPESP's process: 19/08345-3 - Supercritical assisted process for the coencapsulation of curcumin and vitamin D3 into liposomes
• Grantee: Matheus Andrade Chaves
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 17/10954-2 - Enrichment of cornstarch with curcumin and Vitamin D3 coencapsulated in lyophilized liposomes using wet agglomeration by high shear
• Grantee: Matheus Andrade Chaves
• Support Opportunities: Scholarships in Brazil - Doctorate