Trace elements distribution in tropical tree rings through high-resolution imaging using LA-ICP-MS analysis

Author(s)

Amais, Renata S. ^[1] ; Moreau, Pedro S. ^[1] ; Francischini, Danielle S. ^[1] ; Magnusson, Rafael ^[1] ; Locosselli, Giuliano M. ^[2] ; Godoy-Veiga, Milena ^[3] ; Ceccantini, Gregorio ^[3] ; Rodriguez, Daigard R. Ortega ^[4] ; Tomazello-Filho, Mario ^[4] ; Arruda, Marco A. Z. ^[1]

Affiliation

^[1] Univ Estadual Campinas, Inst Chem, Sample Preparat & Mechanizat Grp GEPAM, Spectrometry, POB 6154, BR-13083970 Campinas, SP - Brazil

^[2] Inst Bot, Ave Miguel Stefano, BR-04301902 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Inst Biosci, Dept Bot, Rua Do Matao 277, BR-05508090 Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Dept Forest Sci, Luiz de Queiroz Coll Agr, Piracicaba - Brazil

• Document type: Journal article
• Source: JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY; v. 68, DEC 2021.
• Web of Science Citations: 0

Abstract

Background: The distribution of trace elements in tree rings although poorly known may be useful to better understand environmental changes, pollution trends, long-term droughts, forest dieback processes, and biology of trees. Method: Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is used for imaging micronutrients and potentially toxic elements distribution, allowing the investigation of trace elements at high spatial resolution within the tree rings. To ensure a more efficient determination of micronutrients and potentially toxic elements, LA-ICP-MS instrumental conditions were optimized and carbon, a major element in wood, is used as an internal standard during analysis to correct for random fluctuations. Results: Spatial distributions maps of Ba, Cu, Fe, Mn, Ni, and Pb in growth layers of six tropical tree species were built-up using the LA-iMageS software, namely: Amburana cearensis (Fabaceae), Cedrela fissilis (Meliaceae), Hymenaea courbaril (Fabaceae), Maclura tinctoria (Moraceae), Parapiptadenia zehntneri (Fabaceae), Peltogyne paniculata (Fabaceae). A correlation between the trace element composition and different cell types (parenchyma, fiber, and vessel) was distinctly observed. It was observed a general pattern of Ba, Cu, Ni, Mn, and Pb accumulation mainly in the axial parenchyma and vessels. But the elemental composition of xylem cells is strongly species dependent. The multivariate analysis also points to a distinct accumulation of minerals between heartwood and sapwood in the same species. Conclusions: Imaging both essential and deleterious element distributions in the tree rings may improve visualization and can effectively contribute to understanding the lifetime metabolism of trees and evaluating the effects of environmental changes related to climatic seasonality, pollution, and future paleoclimate reconstructions. (AU)

• FAPESP's process: 19/24445-8 - EMU granted in process 2018/25207-0: inductively coupled plasma mass spectrometer
• Grantee: Marco Aurelio Zezzi Arruda
• Support type: Multi-user Equipment Program
• FAPESP's process: 20/09251-0 - Functional forests: biodiversity in the benefit of cities
• Grantee: Giuliano Maselli Locosselli
• Support type: Scholarships in Brazil - Young Researchers
• FAPESP's process: 13/21728-2 - The use of modern autopsy techniques to investigate human diseases (MODAU)
• Grantee: Paulo Hilário Nascimento Saldiva
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 17/50085-3 - PIRE: climate research education in the Americas using tree-ring speleothem examples (PIRE-CREATE)
• Grantee: Francisco William da Cruz Junior
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 18/22914-8 - Global climate change in the Amazon tropical forest assessed by dendrocronological multi-proxy
• Grantee: Daigard Ricardo Ortega Rodriguez
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/25207-0 - Nanometallomics applied to soybean cultivation for studying the effects of metal-related nanoparticles
• Grantee: Marco Aurelio Zezzi Arruda
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 15/25511-3 - Climate and pollution reconstruction based on urban tree-rings growth records
• Grantee: Giuliano Maselli Locosselli
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 18/23478-7 - Qualitative elemental distribution evaluation and elemental bioimaging through laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to tree rings and speleothems
• Grantee: Renata Stábile Amais
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 19/08783-0 - Functional forests: biodiversity in the benefit of cities
• Grantee: Giuliano Maselli Locosselli
• Support type: Research Grants - Young Investigators Grants
• FAPESP's process: 19/00063-9 - Quantitative bioimaging of Cd and Pb through laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS imaging) to tree rings and speleothems
• Grantee: Danielle da Silva Francischini
• Support type: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 17/50341-0 - Challenges for biodiversity conservation facing climate changes, pollution, land use and occupation (PDIp)
• Grantee: Luiz Mauro Barbosa
• Support type: Research Grants - State Research Institutes Modernization Program
• FAPESP's process: 19/00018-3 - Study of inert supports as solid matrix for the quantification of Zn and Cu in tree rings and speleothems using LA-ICP-MS
• Grantee: Pedro dos Santos Moreau
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 19/05174-3 - Evaluation of sample preparation focusing on the quantification of Cd and Pb through laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to tree rings and speleothems
• Grantee: Rafael Magnusson
• Support type: Scholarships in Brazil - Scientific Initiation