In vivo toxicogenic potential of Salix alba (Salicaceae) bark extract

Author(s)

Maistro, Edson Luis ^{[1, 2]} ; Terrazzas, Peterson Menezes ^[2] ; Helena Frankland Sawaya, Alexandra Christinie ^[3] ; Pires Rosa, Paulo Cesar ^[3] ; Perazzo, Fabio Ferreira ^{[4, 5]} ; de Mascarenhas Gaivao, Isabel O'Neill ^[6]

Affiliation

^[1] Sao Paulo State Univ, Speech & Hearing Therapy Dept, UNESP, Sao Paulo - Brazil

^[2] Sao Paulo State Univ, Inst Biociencias, UNESP, Programa Posgrad Biol Gera & Aplicada, Botucatu, SP - Brazil

^[3] Univ Estadual Campinas, Fac Pharmaceut Sci, Campinas - Brazil

^[4] Univ Tras Os Montes & Alto Douro UTAD, Dept Genet & Biotechnol, Vila Real - Portugal

^[5] Univ Tras Os Montes & Alto Douro UTAD, Anim & Vet Res Ctr CECAV, Vila Real - Portugal

^[6] Univ Tras Os Montes & Alto Douro, Genet Biotech Anim Vet Res Ctr, Escola Ciencias Agr & Vet, Vila Real - Portugal

• Document type: Journal article
• Source: JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES; OCT 2021.
• Web of Science Citations: 0

Abstract

Salix alba (white willow) bark extract is widely used for conditions associated with inflammation, fever, microbial infection or pain. Exposure of human cultured leukocytes to S. alba in vitro noted a genotoxic response. However, data regarding the influence of this bark extract on DNA damage in vivo are lacking. The main goal of this study was to examine the potential of S.alba bark extract to induce DNA damage and chromosome aberrations in an in vivo model using cells obtained from male Swiss albino mice administered the compound orally. The extract was administered by oral gavage daily for 7 days at doses of 500, 1000, or 2000 mg/kg b.w. Genotoxicity analysis was performed using the comet assay on peripheral blood leukocytes, as well as liver, bone marrow, heart, and testicular cells collected 4 hr after the last treatment and the micronucleus (MN) test on bone marrow cells. In essence cells were collected 28 hr after the penultimate treatment Data demonstrated that S. alba bark extract did not induce significant DNA damage in any cell types examined, or clastogenic/aneugenic effects as detected by the MN test at the three tested doses. Under these experimental conditions, evidence indicates that S.alba bark extract did not initiate genotoxic or chromosome aberrations in various mouse cells investigated. (AU)

• FAPESP's process: 17/24149-4 - Study of the cytotoxicity, genotoxicity, cell cycle monitoring, induction of apoptosis, and gene expression of Rubus rosaefolius leaves and stems extracts in human cultured cells.
• Grantee: Edson Luis Maistro
• Support Opportunities: Regular Research Grants