CHX Stabilizes the Resin/demineralized Dentin Interface

Author(s)

Ana Rosa Costa ^[1] ; Lucas Zago Naves ^[2] ; Franklin Garcia-Godoy ^[3] ; Fernanda Midori Tsuzuki ^[4] ; Américo Bortolazzo Correr ^[5] ; Lourenço Correr-Sobrinho ^[6] ; Regina Maria Puppin-Rontani ^[7]

Affiliation

^[1] UNICAMP. Piracicaba Dental School. Dental Materials Division - Brasil

^[2] University Medical Center Groningen. Department of Restorative Dentistry and Biomaterials - Holanda

^[3] University of Tennessee Health Science Center. Department of Bioscience Research - Estados Unidos

^[4] UNICAMP. Piracicaba Dental School. Dental Materials Division - Brasil

^[5] UNICAMP. Piracicaba Dental School. Dental Materials Division - Brasil

^[6] UNICAMP. Piracicaba Dental School. Dental Materials Division - Brasil

^[7] State University of Campinas. Department of Health Sciences, Orthodontics and Pediatric Dentistry, Pediatric Division, Piracicaba Dental School - Brasil

• Document type: Journal article
• Source: Brazilian Dental Journal; v. 32, n. 4, p. 106-115, 2021-11-12.

Abstract

Abstract The aim of this study was to evaluate the effect of 2% chlorhexidine digluconate (CHX) on microtensile bond strength (µTBS) between an adhesive system and under 3 dentin conditions. For that, this study evaluated the adhesive interface at initial, after 6 months and 1 year of storage. Forty-eight human third molars were prepared and randomly divided into 3 groups, according to dentin substrates: sound dentin (Sd), caries-infected dentin (Ci) and caries-affected dentin (Ca). The groups were subdivided into two according to the dentin pre-treatment: application of 2% CHX or without pre-treatment (control). The dentin surfaces were etched with 35% phosphoric acid gel and bonded with Adper Single Bond 2 (3M ESPE) adhesive system according to manufacturer’s instructions. Subsequently, the specimens were stored in deionized water at 37°C for 24h, 6 months and 1 year. Two additional teeth were used to analyze the bonding interfaces by SEM. Data was submitted to three-way ANOVA in a split plot design and Tukey’s test (α = 0.05). The results showed that Ci decreased µTBS values when compared to Ca and Sd, regardless storages time or treatment. Stored samples for 6 months and 1 year decreased the µTBS for the control group, but no difference was found between storages time for the CHX group. As a conclusion, the 2% CHX application after etching showed improved dentin bond strength in the storage time, regardless of the substrates evaluated. (AU)

• FAPESP's process: 10/07829-2 - Study in vitro the effect of chlorhexidine and ethanol on the dentin/resin bond strength at different dentin substrate conditions and storage time
• Grantee: Ana Rosa Costa Correr
• Support Opportunities: Scholarships in Brazil - Doctorate