Analysis of cytotoxicity and genotoxicity in a short-term dependent manner induced by a new titanium dioxide nanoparticle in murine fibroblast cells

Author(s):Show less -

Pedrino, Matheus ^[1] ; Brassolatti, Patricia ^[1] ; Maragno Fattori, Ana Carolina ^[1] ; Bianchi, Jaqueline ^[1] ; de Almeida Rodolpho, Joice Margareth ^[1] ; de Godoy, Krissia Franco ^[1] ; Assis, Marcelo ^[2] ; Longo, Elson ^[2] ; Zambone Pinto Rossi, Karina Nogueira ^[1] ; Speglich, Carlos ^[3] ; Anibal, Fernanda de Freitas ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, Morphol & Pathol Dept, BR-13565905 Sao Carlos - Brazil

^[2] Univ Fed Sao Carlos, Ctr Dev Funct Mat CDMF, Sao Carlos - Brazil

^[3] Leopoldo Americo Miguez Mello Res Ctr CENPES, Rio De Janeiro - Brazil

• Document type: Journal article
• Source: TOXICOLOGY MECHANISMS AND METHODS; NOV 2021.
• Web of Science Citations: 0

Abstract

The extensive use of titanium dioxide nanoparticles (TiO2 NPs) in cosmetics, food, personal care products, and industries brought concerns about their possible harmful effects. Nowadays it has become important to assess TiO2 NPs toxic effects as a way to understand their primary risks. In the cellular environment, after cell uptake, TiO2 NPs were described to induce reactive oxygen species (ROS) production, unbalance oxidative state, and activate apoptosis in several cell lines. Therefore, we aimed to evaluate the cytotoxicity and genotoxicity of a new TiO2 NP surface-functionalized with sodium carboxylic ligands in a murine fibroblast cell line (LA-9). TEM and DLS analyses were performed to define nanoparticle physicochemical characteristics. We evaluated the metabolic activity and LDH released after 24 h exposition to determine cytotoxic effects. Also, we evaluated DNA damage, intracellular reactive oxygen species (ROS) production, and apoptosis induction after 24 h exposure. The TiO2 NP impaired the cell membrane integrity at 1000 mu g/mL, induced intracellular ROS production and late apoptosis at 24 h. The genotoxic effects were observed at all conditions tested at 24 h. Indeed, in fibroblasts exposed at 100 mu g/mL was observed early apoptosis cells. The intracellular ROS content was increased in a dose-dependent manner. Thus, short-term exposure to TiO2 NP promoted cytotoxicity, genotoxicity and activated apoptosis pathways based on the potential role of oxygen species in the fibroblasts cell line. (AU)

• FAPESP's process: 13/07296-2 - CDMF - Center for the Development of Functional Materials
• Grantee: Elson Longo da Silva
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC