Better Response to Influenza Virus Vaccination in Physically Trained Older Adults Is Associated With Reductions of Cytomegalovirus-Specific Immunoglobulins as Well as Improvements in the Inflammatory and CD8(+) T-Cell Profiles

Author(s):Show less -

Felismino, Eduardo S. ^[1] ; Santos, Juliana M. B. ^[2] ; Rossi, Marcelo ^[3] ; Santos, Carlos A. F. ^[4] ; Durigon, Edison L. ^{[5, 6]} ; Oliveira, Danielle B. L. ^{[7, 6]} ; Thomazelli, Luciano M. ^[6] ; Monteiro, Fernanda R. ^{[3, 8]} ; Sperandio, Adriane ^[8] ; Apostolico, Juliana S. ^[9] ; Franca, Carolina N. ^[1] ; Amaral, Jonatas B. ^[3] ; Amirato, Gislene R. ^[3] ; Vieira, Rodolfo P. ^{[10, 11, 2]} ; Vaisberg, Mauro ^[3] ; Bachi, Andre L. L. ^{[11, 3, 1]}

Affiliation:Show less -

^[1] Univ Santo Amaro, Postgrad Program Hlth Sci, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Postgrad Program Sci Human Movement & Rehabil, Santos, SP - Brazil

^[3] Univ Fed Sao Paulo, Dept Otorhinolaryngol, Ear Nose & Throat ENT Lab, Sao Paulo - Brazil

^[4] Paulista Sch Med EPM, Dept Med, Geriatry, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Sci Platform Pasteur, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Lab Clin & Mol Virol, Sao Paulo - Brazil

^[7] Hosp Israelita Albert Einstein, Sao Paulo - Brazil

^[8] Method Fac Sao Paulo, Sao Paulo - Brazil

^[9] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil

^[10] Univ Brasil, Postgrad Program Bioengn, Sao Paulo - Brazil

^[11] Brazilian Inst Teaching & Res Pulm & Exercise Imm, Sao Jose Dos Campos - Brazil

• Document type: Journal article
• Source: FRONTIERS IN IMMUNOLOGY; v. 12, OCT 12 2021.
• Web of Science Citations: 0

Abstract

Chronic cytomegalovirus (CMV) infection is a trigger factor for the development of immunosenescence and negatively impacts the immune response to influenza virus vaccination (IVV) in older adults. However, the role of physical exercise training in this context is unknown. Thus, the aim of this study was to investigate whether the regular practice of combined exercise training can improve the specific antibody response to IVV in CMV-seropositive older adults. Eighty older adults were distributed into two groups-non-practitioners (NP, n = 31, age = 74.06 +/- 6.4 years) and practitioners of combined exercise training (CET, n = 49, age = 71.7 +/- 5.8 years)-for at least 12 months. Both volunteer groups were submitted to IVV and blood samples were collected before (pre) and 30 days after (post) the vaccination. Concerning the specific antibody response to IVV, higher serum levels of specific immunoglobulin A (IgA) were found in the CET group post- than pre-vaccination (p < 0.01), whereas higher levels of specific immunoglobulin M (IgM) were observed both in the NP (p < 0.05) and CET (p < 0.001) groups post-vaccination as compared to the pre-vaccination values. Serum levels of specific immunoglobulin G (IgG) for IVV and CMV, as well as interleukin 6 (IL-6) and IL-10, were similar between the time points evaluated. However, the IL-10/IL-6 ratio post-vaccination was higher (p < 0.05) in the CET group than that before vaccination. Negative correlations were observed between the specific IgG levels for IVV and CMV only in the CET group, both pre- and post-vaccination. In addition, negative correlations were found between IL-10 and specific IgG for CMV in all volunteer groups pre- and post-vaccination, whereas a positive correlation between IL-10 and specific-IgG for IVV pre- and post-vaccination was observed in the CET group. In addition, with the hemagglutination inhibition (HAI) assay, it was found that 32.2% of the NP group and 32.6% of the CET group were responders to IVV and displayed reductions in the CMV serostatus (p < 0.05 and p < 0.001, respectively) and increases in naive and effector CD8(+) T cells post-vaccination (p < 0.01). However, only the responders from the CET group showed significant reductions in the ratio of effector to naive CD8(+) T cells (p < 0.05) and increased IL-10 levels post-vaccination (p < 0.001). In summary, this study demonstrates that the improvement in the response to IVV in CMV-seropositive older adults was related to an anti-inflammatory status and enhancement of naive CD8(+) T cells, particularly associated with regular practice of CET.</p> (AU)

• FAPESP's process: 20/06409-1 - Evaluation of humoral immune response and inflammatory response in patients with confirmed diagnosis of COVID-19 at Hospital Sírio Libanês and correlation with disease severity
• Grantee: Edison Luiz Durigon
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/20045-7 - Antigen discovery and development of serological diagnostic methods and vaccine approaches against the Zika Virus (ZIKV)
• Grantee: Luis Carlos de Souza Ferreira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals
• Grantee: Rodolfo de Paula Vieira
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 19/14115-0 - Influence of L-glutamine supplementation in the sarcopenia and immune/inflammatory response both systemic and in the respiratory tract of elderly practitioners or not of a regular program of combined physical exercises submitted to Influenza virus vaccine
• Grantee: André Luis Lacerda Bachi
• Support Opportunities: Regular Research Grants