| Full text | |
| Author(s): |
Minanni, Carlos Andre
[1, 2]
;
Machado-Lima, Adriana
[3, 1]
;
Iborra, Rodrigo Tallada
[1, 4]
;
Okuda, Ligia Shimabukuro
[1]
;
Pinto, Raphael de Souza
[1]
;
Mello Santana, Monique de Fatima
[1]
;
de Araujo Lira, Aecio Lopes
[1]
;
Nakandakare, Edna Regina
[1]
;
Cardillo Correa-Giannella, Maria Lucia
[5]
;
Passarelli, Marisa
[6, 1]
Total Authors: 10
|
| Affiliation: | [1] Univ Sao Paulo, Lab Lipides LIM 10, Hosp Clin HCFMUSP, Fac Med, BR-01246000 Sao Paulo - Brazil
[2] Hosp Israelita Albert Einstein HIAE, BR-05652900 Sao Paulo - Brazil
[3] Univ Sao Judas Tadeu, Programa Posgrad Ciencias Envelhecimento, BR-03166000 Sao Paulo - Brazil
[4] Univ Sao Judas Tadeu, Programa Posgrad Educ Fis, BR-03166000 Sao Paulo - Brazil
[5] Univ Sao Paulo, Lab Carboidratos & Radioimunoensaio LIM 18, Hosp Clin HCFMUSP, Fac Med, BR-01246000 Sao Paulo - Brazil
[6] Univ Nove de Julho, Programa Posgrad Med, BR-01525000 Sao Paulo - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | NUTRIENTS; v. 13, n. 10 OCT 2021. |
| Web of Science Citations: | 1 |
| Abstract | |
Advanced glycated albumin (AGE-albumin) impairs cholesterol efflux and contributes to inflammation in macrophages. The current study evaluated: (1) the persistence of the deleterious effect of AGE-albumin in cholesterol efflux and in inflammation, and (2) how metabolic control in diabetes mellitus (DM) contributes to attenuate the deleterious role of AGE-albumin in macrophage cholesterol homeostasis. Methods: AGE-albumin was produced in vitro or isolated from uncontrolled DM subjects' serum before (bGC) and after improved glycemic control (aGC). Albumin samples were incubated with bone marrow-derived macrophages and C-14-cholesterol efflux or LPS- induced cytokine secretion were determined immediately, or after cell resting in culture media alone. The ABCA-1 degradation rate was determined after cell incubation with cycloheximide, and ABCA1 protein level by immunoblot. Oil Red O staining was used to assess intracellular lipid accumulation. Results: A persistent effect of AGE-albumin was observed in macrophages in terms of the secretion of inflammatory cytokines and reduced cholesterol efflux. HDL-mediated C-14-cholesterol efflux was at least two times higher in macrophages treated with aCG-albumin as compared to bGC-albumin, and intracellular lipid content was significantly reduced in aGC-albumin-treated cells. As compared to bGC-albumin, the ABCA-1 protein content in whole cell bulk was 94% higher in aCG-albumin. A 20% increased ABCA-1 decay rate was observed in macrophages treated with albumin from poorly controlled DM. AGE-albumin has a persistent deleterious effect on macrophage lipid homeostasis and inflammation. The reduction of AGEs in albumin ameliorates cholesterol efflux.</p> (AU) | |
| FAPESP's process: | 17/18545-4 - HDL proteomics and functionality in diabetes mellitus chronic kidney disease: association with advanced glycation end products |
| Grantee: | Monique de Fatima Mello Santana |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 12/19112-0 - Involvement of the RAGE/AGE axis in macrophage lipid accumulation induced by human advanced glycated albumin - contribution of glycemic control in diabetes mellitus. |
| Grantee: | Adriana Machado Saldiba de Lima |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 16/15603-0 - Unraveling mechanisms of glycemic control and chronic complications of Diabetes mellitus: contributions to human health |
| Grantee: | Ubiratan Fabres Machado |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 12/12088-7 - Glycemic control and the removal of macrophage cholesterol by ABCA-1: role of modified albumin by advanced glycation |
| Grantee: | Rodrigo Tallada Iborra |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/00172-0 - Advanced glycation products, endoplasmic reticulum stress and the modulation of the reverse cholesterol transport in human arteries with different levels Atherosclerotic lesion |
| Grantee: | Raphael de Souza Pinto |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 15/21072-5 - Influence of the glycemic control and nephropathy stage on the reverse cholesterol transport in Diabetes mellitus: role of metabolic memory induced by advanced glycated albumin |
| Grantee: | Marisa Passarelli |
| Support Opportunities: | Regular Research Grants |