Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Estrogen deficiency during puberty affects the expression of microRNA30a and microRNA503 in the mandibular condyle

Full text
Author(s):
Bergamo, Ana Zilda Nazar [1] ; Madalena, Isabela Ribeiro [1, 2, 3] ; Omori, Marjorie Ayumi [1] ; Ramazzotto, Lucas Alexandre [1, 4] ; Nelson-Filho, Paulo [1] ; Baratto-Filho, Flares [2] ; Proff, Peter [5] ; Kirschneck, Christian [5] ; Kuechler, Erika Calvano [5]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Pediat Clin, Ribeirao Preto, SP - Brazil
[2] Univ Region Joinville, Dept Dent, Joinville, SC - Brazil
[3] Univ Fed Juiz de Fora, Dept Restorat Dent, Juiz De Fora, MG - Brazil
[4] Univ Fed Sao Carlos, Biotechnol Grad, Sao Carlos, SP - Brazil
[5] Univ Med Ctr Regensburg, Dept Orthodont, Regensburg - Germany
Total Affiliations: 5
Document type: Journal article
Source: ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER; v. 240, FEB 2022.
Web of Science Citations: 0
Abstract

Background: The aim of this study was investigated if estrogen deficiency during puberty affects the expression of miRNA30a and miRNA503 in maxillary and mandibular growth centers, and also evaluated if ER alpha and ER beta are correlated with miRNA30a and miRNA503 expressions. Methods: Samples from 12 female Wistar rats randomized into experimental group (OVX) and control group (SHAM). At an age of 45 days animals were euthanized for miRNA expression analyses. RT-qPCR was performed to determine miRNA30a and miRNA503 expression in growth sites: midpalatal suture, condyle, mandibular angle, symphysis/parasymphysis and coronoid process. The data was carried out using the parametric tests at 5% of significance level. Results: miRNA 30a and miRNA503 presented higher levels in the condylar site in SHAM group when compared with OVX (p = 0.002 and p = 0.020, respectively). In the growth centers, a statistical significant difference was observed only for miRNA30a (p = 0.004), when compared mandibular angle with condyle the in OVX group (p = 0.001). A strong positive correlation between miRNA503 and ER alpha in the condyle of OVX group was observed (r = 0.90; p = 0.039 and it also between miRNA503 and ER beta in the coronoid process of the OVX group (r = 0.88; p = 0.05). Conclusion: The results suggested that estrogen regulates specific miRNAs in maxillary and mandibular growth centers, which may participate in posttranscriptional regulation of estrogen-regulated genes. (c) 2021 Published by Elsevier GmbH. (AU)

FAPESP's process: 15/06866-5 - Evaluation of the role of estrogen in dentofacial development
Grantee:Erika Calvano Kuchler
Support Opportunities: Research Grants - Young Investigators Grants