Cationic and Biocompatible Polymer/Lipid Nanoparticles as Immunoadjuvants

Author(s)

Perez-Betancourt, Yunys ^[1] ; Araujo, Pericles Marques ^[1] ; Lins Fernandes Tavora, Bianca de Carvalho ^{[2, 3]} ; Pereira, Daniele Rodrigues ^{[2, 3]} ; Faquim-Mauro, Eliana Lima ^{[2, 3]} ; Carmona-Ribeiro, Ana Maria ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, Biocolloids Lab, Ave Prof Linen Prestes 748, BR-05508000 Sao Paulo - Brazil

^[2] Butantan Inst, Immunopathol Lab, Ave Vital Brasil 1500, BR-05503900 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, Ave Prof Lineu Prestes 1730, BR-05508000 Sao Paulo - Brazil

• Document type: Journal article
• Source: PHARMACEUTICS; v. 13, n. 11 NOV 2021.
• Web of Science Citations: 0

Abstract

Nanostructures have been of paramount importance for developing immunoadjuvants. They must be cationic and non-cytotoxic, easily assembling with usually oppositely charged antigens such as proteins, haptens or nucleic acids for use in vaccines. We obtained optimal hybrid nanoparticles (NPs) from the biocompatible polymer poly(methyl methacrylate) (PMMA) and the cationic lipid dioctadecyl dimethyl ammonium bromide (DODAB) by emulsion polymerization of methyl methacrylate (MMA) in the presence of DODAB. NPs adsorbed ovalbumin (OVA) as a model antigen and we determined their adjuvant properties. Interestingly, they elicited high double immune responses of the cellular and humoral types overcoming the poor biocompatibility of DODAB-based adjuvants of the bilayer type. The results suggested that the novel adjuvant would be possibly of use in a variety of vaccines. (AU)

• FAPESP's process: 19/17685-2 - Supramolecular assemblies with immunoadjuvant or antimicrobial activity
• Grantee: Ana Maria Carmona-Ribeiro
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/17625-0 - Study of the molecular mechanisms involved in the immunomodulation exerted by crotoxin and phospholipase A2 from Crotalus durissus terrificus venom
• Grantee: Eliana Faquim de Lima Mauro
• Support Opportunities: Regular Research Grants