rotective effect of green tea and epigallocatechin-3-gallate in a LPS-induced systemic inflammation mode

Author(s)

Azambuja, Juliana Hofstatter ^[1] ; Mancuso, Rubia Isler ^[1] ; Della Via, Fernanda Isabel ^[1] ; Torello, Cristiane Okuda ^[1] ; Saad, Sara Teresinha Olalla ^[1]

Affiliation

^[1] Univ Estadual Campinas, Hematol & Transfus Med Ctr, Rua Carlos Chagas 480, BR-13083878 Campinas, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF NUTRITIONAL BIOCHEMISTRY; v. 101, MAR 2022.
• Web of Science Citations: 0

Abstract

Y Inflammation causes severe dysregulation of organ functions, via the development of oxidative stress and inflammation damage. Polyphenol compounds found in green tea (GTE), including the most important component epigallocatechin-3-gallate (EGCG), have a great therapeutic potential. Here, protective properties of GTE and EGCG against lipopolysaccharide (LPS)-induced inflammation are explored. To this end, the effects of GTE and EGCG were studied on LPS challenged macrophages. Mice received GTE (250 mg/kg/d/p.o) or EGCG (25 mg/kg/d/i.p.) for 7 d, before the inflammation shock was provoked with a single intraperitoneal injection of LPS. The frequencies of lymphocytes CD4(+), CD8(+), NK1-1(+) and CD4 (+)CD25(high)FOXP3(+) (Treg), macrophages CD11b(+) F480(+), monocytes CD11b(+)Ly6C(low/high), neutrophils CD11b(+)Ly6G(+), MDSCs CD11b(+)Gr-1(high), M2/N2-like phenotype CD206(+) and M1-like phenotype CD86(+) in spleen, bone marrow and peripheral blood were determined. In vitro studies revealed that GTE and EGCG significantly attenuated LPS-induced CD80 expression and increased the CD163 expression, showing a potential to reduce the macrophage inflammatory phenotype. In vivo , GTE and EGCG inhibited the inflammation, mainly by reducing M1-macrophages and increasing Treg cells in the bone marrow. In addition, GTE and EGCG increase M2-macrophages, N2-neutrophils and Tregs in the spleen and blood and block the migration of monocytes from the bone marrow to the peripheral blood. These findings indicate that EGCG and GTE prevent LPS-induced inflammatory damage contributing to restoring the immune system homeostasis (C) 2021 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 17/21801-2 - Predictors of severity and new treatments for bone marrow neoplasias
• Grantee: Sara Teresinha Olalla Saad
• Support Opportunities: Research Projects - Thematic Grants