Impact of irrigation protocols with some chelators and mechanical agitation on intratubular decontamination

Author(s)

Pedrinha, Victor Feliz ^[1] ; Cardenas Cuellar, Maricel Rosario ^[2] ; Velasquez-Espedilla, Evelyn Giuliana ^[2] ; Hungaro Duarte, Marco Antonio ^[2] ; de Andrade, Flaviana Bombarda ^[2] ; Rodrigues, Patricia de Almeida ^[1]

Affiliation

^[1] Univ Fed Para UFPA, Lab Dent Mat, Grad Program Dent, Belem, Para - Brazil

^[2] Univ Sao Paulo, Bauru Dent Sch, Dept Operat Dent Dent Mat & Endodont, Bauru, SP - Brazil

• Document type: Journal article
• Source: Brazilian Oral Research; v. 35, 2021.
• Web of Science Citations: 0

Abstract

Abstract: This study evaluated main canal and intratubular decontamination using different irrigation solutions followed by adjunctive agitation steps for infected root canals. Sixty-eight lower incisors were contaminated with Enterococcus faecalis and allocated to groups according to canal treatment (n = 10): G1, NaOCl followed by ethylenediaminetetraacetic acid (EDTA); G2, a mixture of NaOCl with hydroxyethylidene bisphosphonate (HEBP); and G3, NaOCl followed by EDTA-T (EDTA with sodium lauryl ether sulfate). All three groups of teeth were agitated with passive ultrasonic irrigation (PUI) using saline solution, whereby G4, G5, and G6 were prepared as above, and agitation was performed using an XP-Endo Finisher instrument. Microbiological samples were collected from the root canals with paper points at three times: before and after chemomechanical preparation and after agitation. The colony-forming units (CFU)/mL count was determined, and bacterial intratubular viability was analyzed via confocal laser scanning microscopy using Live/Dead staining. Statistical analysis was performed using a Kruskal-Wallis test followed by Dunn tests. A Friedman test was applied for colony-counting data (α = 0.05). CFU/mL counting indicated equally effective decontamination in the experimental groups (p > 0.05). According to microscopy images, the use of irrigation solutions followed by agitation with the XP-Endo Finisher yielded better results. Moreover, NaOCl+EDTA-T followed by XP-Endo Finisher resulted in significantly lower viability than in the PUI-activated groups (p < 0.05). The cervical and medium thirds of the specimens presented similar results. Overall, NaOCl+EDTA-T exhibited the best intratubular antibacterial activity, mainly for canals that were subsequently agitated using XP-Endo Finisher. (AU)

• FAPESP's process: 10/20186-3 - Adherence factors and biofilms generated by root canal microorganisms
• Grantee: Flaviana Bombarda de Andrade
• Support Opportunities: Regular Research Grants