ialidase Activity in the Cervicovaginal Fluid Is Associated With Changes in Bacterial Components of Lactobacillus-Deprived Microbiot

Author(s)

Ferreira, Carolina Sanita Tafner ^[1] ; Marconi, Camila ^{[1, 2]} ; Parada, Cristina M. G. L. ^[3] ; Ravel, Jacques ^[4] ; Silva, Marcia Guimaraes da ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Botucatu Med Sch, Dept Pathol, Botucatu, SP - Brazil

^[2] Fed Univ Parana UFPR, Dept Basic Pathol, Sect Biol Sci, Curitiba, Parana - Brazil

^[3] Sao Paulo State Univ UNESP, Botucatu Med Sch, Dept Nursing, Botucatu, SP - Brazil

^[4] Univ Maryland, Sch Med, Inst Genom Sci, Baltimore, MD 21201 - USA

• Document type: Journal article
• Source: FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY; v. 11, JAN 13 2022.
• Web of Science Citations: 0

Abstract

IntroductionSialidase activity in the cervicovaginal fluid (CVF) is associated with microscopic findings of bacterial vaginosis (BV). Sequencing of bacterial 16S rRNA gene in vaginal samples has revealed that the majority of microscopic BV cases fit into vaginal community-state type IV (CST IV), which was recently named ``molecular-BV.{''} Bacterial vaginosis-associated bacterial species, such as Gardnerella spp., may act as sources of CVF sialidases. These hydrolases lead to impairment of local immunity and enable bacterial adhesion to epithelial and biofilm formation. However, the impact of CVL sialidase on microbiota components and diversity remains unknown. ObjectiveTo assess if CVF sialidase activity is associated with changes in bacterial components of CST IV. MethodsOne hundred forty women were cross-sectionally enrolled. The presence of molecular-BV (CST IV) was assessed by V3-V4 16S rRNA sequencing (Illumina). Fluorometric assays were performed using 2-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid (MUAN) for measuring sialidase activity in CVF samples. Linear discriminant analysis effect size (LEfSe) was performed to identify the differently enriched bacterial taxa in molecular-BV according to the status of CVF sialidase activity. ResultsForty-four participants (31.4%) had molecular-BV, of which 30 (68.2%) had sialidase activity at detectable levels. A total of 24 bacterial taxa were enriched in the presence of sialidase activity, while just two taxa were enriched in sialidase-negative samples. ConclusionSialidase activity in molecular-BV is associated with changes in bacterial components of the local microbiome. This association should be further investigated, since it may result in diminished local defenses against pathogens. (AU)

• FAPESP's process: 12/10403-2 - Characterization of the vaginal microbiome of Brazilian women of reproductive age
• Grantee: Camila Marconi
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 12/16800-3 - Characterization of the vaginal microbiome of Brazilian women in reproductive age
• Grantee: Márcia Guimarães da Silva
• Support Opportunities: Regular Research Grants