accharomyces cerevisiae Dehydrated Culture Modulates Fecal Microbiota and Improves Innate Immunity of Adult Dog

Author(s):Show less -

Santos, Karine de Melo ^[1] ; Risolia, Larissa Wuensche ^[1] ; Rentas, Mariana Fragoso ^[1] ; Amaral, Andressa Rodrigues ^[2] ; Rodrigues, Roberta Bueno Ayres ^[1] ; Urrego, Maria Isabel Gonzalez ^[1] ; Vendramini, Thiago Henrique Annibale ^[1] ; Ventura, Ricardo Vieira ^[1] ; Balieiro, Julio Cesar de Carvalho ^[1] ; Massoco, Cristina de Oliveira ^[1] ; Santos, Joao Paulo Fernandes ^[1] ; Pontieri, Cristiana Fonseca Ferreira ^[3] ; Brunetto, Marcio Antonio ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Nutr & Prod Dept, Pet Nutrol Res Ctr, BR-13635900 Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Vet Nutrol Serv, Vet Teaching Hosp, Sch Vet Med & Anim Sci, Cidade Univ, BR-05508270 Sao Paulo, SP - Brazil

^[3] Grandfood Ind & Comercio LTDA Premier Pet, Nutr Dev Ctr, BR-13590000 Dourado, SP - Brazil

• Document type: Journal article
• Source: ERMENTATION-BASE; v. 8, n. 1 JAN 2022.
• Web of Science Citations: 0

Abstract

Saccharomyces cerevisiae yeast culture can be dehydrated, and it has a potential prebiotic effect. This study evaluated the effects of supplementing increasing levels of dehydrated yeast culture (DYC) of Saccharomyces cerevisiae (Original XPC (TM), Diamond V, Cedar Rapids, IA, USA) on fecal microbiota, nutrient digestibility, and fermentative and immunological parameters of healthy adult dogs. Eighteen adult male and female dogs with a mean body weight of 15.8 +/- 7.37 kg were randomly assigned to three experimental treatments: CD (control diet), DYC 0.3 (control diet with 0.3% DYC) and DYC 0.6 (control diet with 0.6% DYC). After 21 days of acclimation, fecal samples were collected for analysis of nutrient digestibility, microbiota and fecal fermentation products. On the last day, the blood samples were collected for the analysis of immunological parameters. The microbiome profile was assessed by the Illumina sequencing method, which allowed identifying the population of each bacterial phylum and genus. The statistical analyses were performed using the SAS software and the Tukey test for multiple comparison (p < 0.05). Our results suggest that the addition of DYC increased the percentage of the phyla Actinobacteria and Firmicutes (p = 0.0048 and p < 0.0001, respectively) and reduced that of the phylum Fusobacteria (p = 0.0008). Regardless of the inclusion level, the yeast addition promoted reduction of the genera Allobaculum and Fusobacterium (p = 0.0265 and p = 0.0006, respectively) and increased (p = 0.0059) that of the genus Clostridium. At the highest prebiotic inclusion level (DYC 0.6), an increase (p = 0.0052) in the genus Collinsella and decrease (p = 0.0003) in Prevotella were observed. Besides that, the inclusion of the additive improved the apparent digestibility of the crude fiber and decreased the digestibility of crude protein, nitrogen-free extract and metabolizable energy (p < 0.05). There were no significant changes in the production of volatile organic compounds. However, an increase in propionate production was observed (p = 0.05). In addition, the inclusion of yeast resulted in an increased phagocytosis index in both treatments (p = 0.01). The addition of 0.3 and 0.6% DYC to the diet of dogs wase able to modulate the proportions of some phyla and genera in healthy dogs, in addition to yielding changes in nutrient digestibility, fermentative products and immunity in healthy adult dogs, indicating that this additive can modulate fecal microbiota and be included in dog nutrition. (AU)

• FAPESP's process: 15/05493-0 - Effects of including increasing levels of prebiotics in the adult dogs diet on the digestive parameters, fecal fermentation, microbiota and immunity
• Grantee: Karine de Melo Santos
• Support Opportunities: Scholarships in Brazil - Master