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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development and Characterization of a Hydrogel Containing Silver Sulfadiazine for Antimicrobial Topical Applications. Part II: Stability, Cytotoxicity and Silver Release Patterns

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Author(s):
Lilian Vieira Vaz [1] ; Victor Manuel Balcão ; José Martins Oliveira Jr [3] ; Matthieu Tubino [4] ; Angela Jozala [5] ; Valquíria Miwa Hanai Yoshida [6] ; Marta Maria Duarte Carvalho Vila [7]
Total Authors: 7
Affiliation:
[1] University of Sorocaba. PhageLab- Laboratory of Biofilms and Bacteriophages - Brasil
[3] University of Sorocaba. PhageLab- Laboratory of Biofilms and Bacteriophages - Brasil
[4] University of Campinas. Institute of Chemistry - Brasil
[5] University of Sorocaba. PhageLab- Laboratory of Biofilms and Bacteriophages - Brasil
[6] University of Sorocaba. PhageLab- Laboratory of Biofilms and Bacteriophages - Brasil
[7] University of Sorocaba. PhageLab- Laboratory of Biofilms and Bacteriophages - Brasil
Total Affiliations: 7
Document type: Journal article
Source: Brazilian Journal of Pharmaceutical Sciences; v. 58, 2022-03-14.
Abstract

Abstract Hydrogels are interesting for use in the treatment of topical wounds due to their virtually zero toxicity, and capacity for extended release of pharmaceuticals. Silver sulfadiazine (SSDZ) is the drug of choice in the treatment of skin burns. The aim of the study was to determine cytotoxicity, antimicrobial activity and stability of a PVA hydrogel with integrated silver sulfadiazine. SSDZ-hydrogels were prepared using 10% (w/w) PVA (either 89% or 99% hydrolyzed) and 1% (w/w) silver sulfadiazine. Cellular viability was assessed via MTS assays, antimicrobial activity via disk-diffusion and accelerated stability tests were carried out with analysis at 0, 30, 60, 90 and 180 days of storage at 40 ± 2 °C and a relative humidity of 75 ± 5%. The parameters evaluated included organoleptic characteristics, moisture, swelling ability, mechanical strength, FTIR, XRD, TGA and DSC, and silver release patterns via XRD and potentiometry. Cell viability tests indicated some cytotoxicity, although within acceptable levels. After 90 days of storage, SSDZ hydrogel samples exhibited a brown coloration, probably due to the formation of Ag or Ag2O nanoparticles. The SSDZ-loaded hydrogels suffered visual and physical changes; however, these changes did not compromise its use as occlusive wound dressings or its antimicrobial properties. (AU)

FAPESP's process: 16/12234-4 - TransAppIL - Transdermal delivery of structurally and functionally stabilized protein entities applying ionic liquids.
Grantee:Marta Maria Duarte Carvalho Vila
Support Opportunities: Regular Research Grants
FAPESP's process: 18/05522-9 - PsaPhageKill - isolation, characterisation and use of lytic bacteriophages against Pseudomonas syringae PV. actinidiae to fight the cancer of kiwifruit: an efficient and ecofriendly alternative
Grantee:Vitor Manuel Cardoso Figueiredo Balcão
Support Opportunities: Scholarships abroad - Research
FAPESP's process: 16/08884-3 - PneumoPhageColor - development of a colorimetric biodetection kit for Pseudomonas aeruginosa based on phage particles
Grantee:Vitor Manuel Cardoso Figueiredo Balcão
Support Opportunities: Regular Research Grants