Unveiling Xylanolytic Enzymes Production of Talaromyces wortmannii DR49 on Industrial Agro Wastes

Author(s)

Diogo Robl ^[1] ; Vania Vicente ^[2] ; Ida Chapaval Pimentel ^[3] ; Gabriel Padilla ^[4] ; José Geraldo da Cruz Pradella ^[5]

Affiliation

^[1] Federal University of Santa Catarina. Department of Microbiology, Immunology and Parasitology - Brasil

^[2] Federal University of Paraná. Department of Basic Pathology - Brasil

^[3] Federal University of Paraná. Department of Basic Pathology - Brasil

^[4] University of São Paulo. Department of Microbiology - Brasil

^[5] Federal University of Sao Paulo. Department of Science and Technology - Brasil

• Document type: Journal article
• Source: Brazilian Archives of Biology and Technology; v. 64, 2022-02-21.

Abstract

Abstract Xylan degradation is an important step in different industries, such as in biorefinery for biomass hydrolysis. Talaromyces wortmannii is a known fungus due to second metabolite production but only few works showed the xylanolytic potential of this fungus. In this way, the aim of this study was to evaluate the production of xylanolytic enzymes from T. wortmannii DR49 on industrial agro wastes. Cultivation in shake flask showed highest xylanase titration (10.3 U/mL; 9.5 U/mL) for wheat bran (WB) and hydrothermal pretreated sugar cane bagasse (HB); in β-xylosidase production WB and xylose were the best carbon sources (0.57 U/mL; 0.34 U/mL) respectively. STR cultivation revealed that 29°C and pH 6.0 were the best conditions for xylanase (14.5 U/mL) and β-xylosidase (1.7 U/mL) production. T. wortmannii DR49 showed to be a potential candidate for xylanolytic enzymes production using agro wastes in bioreactors, which has never been previously reported in this fungus. (AU)

• FAPESP's process: 11/10834-0 - Hemicellulases and accessory proteins of filamentous fungi and actinomycetes for deconstruction of lignocellulosic biomass
• Grantee: Diogo Robl
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)