Synthesis and characterization of a novel silica-niobia support for the immobilization of microbial fructosyltransferase aiming at fructooligosaccharides production

Author(s)

Garcia, Leonardo Abreu ; Morales, Sergio Andres Villalba ; Xavier, Michelle da Cunha Abreu ; Lopes, Melina Savioli ; Silva, Elda Sabino da ; Maiorano, Alfredo Eduardo ; Gunnewiek, Rodolfo Foster Klein ; Perna, Rafael Firmani

• Document type: Journal article
• Source: MATERIALS TODAY COMMUNICATIONS; v. 31, p. 8-pg., 2022-06-01.

Abstract

A new support for fructosyltransferase (FTase, E.C. 2.4.1.9) immobilization was obtained by a facile niobium deposition on powdered silica-gel, using a simple polymeric solution-based method, aiming to obtain a highly active biocatalyst for the production of fructooligosaccharides (FOS). The support was characterized by XRD, SEM, N-2 physisorption, FTIR, TGA and DSC analyses. The support showed orthorhombic and monoclinic Nb2O5 phases homogeneously distributed along the sample. The thermal decomposition of the organics yielded very fine particle sizes of a mixture of silica-niobia material with specific surface area of approximately 127 m(2) g(-1). FTIR analyses confirmed the presence of the immobilized enzyme. The immobilization yield and recovered activity of the enzyme were about 57.7% and 87.8%, respectively. It suggests a significant improvement in FTase adsorption on silica due to the addition of niobium and a high applicability of silica-niobia supports for FTase immobilization aiming at FOS production. (AU)

• FAPESP's process: 17/13769-1 - Multiferroic and ferroelectric materials for energy converters: synthesis, properties, phenomenology and applications
• Grantee: José Antonio Eiras
• Support Opportunities: Research Projects - Thematic Grants