Understanding the role of electrostatic interactions on the association of 5-fluorouracil to chitosan-TPP nanoparticles

Author(s)

dos Santos, Aline Martins ; Carvalho, Suzana Goncalves ; Barboza Ferreira, Leonardo Miziara ; Chorilli, Marlus ; Daflon Gremiao, Maria Palmira

• Document type: Journal article
• Source: COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS; v. 640, p. 8-pg., 2022-05-05.

Abstract

Judicious design strategies should be considered powerful tools to better control the properties of chitosan (CS) and tripolyphosphate (TPP) particles. This study focused on understanding the role of the charge properties of 5-fluorouracil (5-FU) on the formation and properties of CS-TPP nanoparticles. The effect of synthesis variables of CS-TPP nanoparticles (CS to TPP ratio and order of addition) under pH 4.5 and 5.5 was evaluated, where CS and TPP were charged, and 5-FU had two different charges (positive and negative), maximizing the interactions between them for the formation of nanoparticles. The order of addition and drug location resulted in changes in the interaction forces, influencing the particle size and size distribution. 5-FU addition into CS resulted in stronger interactions, favoring the formation of smaller particles. The pH of reactional medium and CS to TPP ratio affected the properties of particles, in terms of size and potential zeta, which led to greater 5-FU entrap-ment. These results demonstrated that the particle properties still depends of the synthesis factors, but the physicochemical properties of drug also influence the reactions/interactions involved in the formation of CS-TPP nanoparticles. (AU)

• FAPESP's process: 14/50928-2 - INCT 2014: Pharmaceutical Nanotechnology: a transdisciplinary approach
• Grantee: Maria Vitória Lopes Badra Bentley
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/01464-9 - Multifunctionalized systems-based chitosan nanoparticles anchored with methotrexate and incorporated into resistant starch/pectin microparticles for colon-specific release of 5-fluourouracil
• Grantee: Aline Martins dos Santos
• Support Opportunities: Scholarships in Brazil - Doctorate