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Graphene-based hybrid electrical-electrochemical point-of-care device for serologic COVID-19 diagnosis

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Mattioli, Isabela A. ; Castro, Karla R. ; Macedo, Lucyano J. A. ; Sedenho, Graziela C. ; Oliveira, Mona N. ; Todeschini, Iris ; Vitale, Phelipe M. ; Ferreira, Suzete Cleusa ; Manuli, Erika R. ; Pereira, Geovana M. ; Sabino, Ester C. ; Crespilho, Frank N.
Total Authors: 12
Document type: Journal article
Source: BIOSENSORS & BIOELECTRONICS; v. 199, p. 10-pg., 2022-03-01.

The outbreak of COVID-19 pandemics highlighted the need of sensitive, selective, and easy-to-handle biosensing devices. In the contemporary scenario, point-of-care devices for mass testing and infection mapping within a population have proven themselves as of primordial importance. Here, we introduce a graphene-based Electrical-Electrochemical Vertical Device (EEVD) point-of-care biosensor, strategically engineered for serologic COVID-19 diagnosis. EEVD uses serologic IgG quantifications on SARS-CoV-2 Receptor Binding Domain (RBD) bioconjugate immobilized onto device surface. EEVD combines graphene basal plane with high charge carrier mobility, high conductivity, low intrinsic resistance, and interfacial sensitivity to capacitance alterations. EEVD application was carried out in real human serum samples. Since EEVD is a miniaturized device, it requires just 40 mu L of sample for a point-of-care COVID-19 infections detection. When compared to serologic assays such ELISA and other immunochromatographic methods, EEVD presents some advantages such as time of analyses (15 min), sample preparation, and a LOD of 1.0 pg mL(-1). We glimpse that EEVD meets the principles of robustness and accuracy, desirable analytic parameters for assays destined to pandemics control strategies. (AU)

FAPESP's process: 17/20493-2 - Study of metalloenzymes through electrochemistry coupled to vibrational spectroscopy
Grantee:Lucyano Jefferson Alves de Macêdo
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 20/04796-8 - In situ and operando studies of metaloenzymes for energy conversion and fuel bioelectrosynthesis
Grantee:Graziela Cristina Sedenho
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 18/11071-0 - Development and application of disposable screen-printed composite electrodes based on metallic nanoparticles and graphite-polyurethane
Grantee:Isabela Alteia Mattioli
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
Grantee:Osvaldo Novais de Oliveira Junior
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 19/12053-8 - High performance electrodes applied in organic batteries and in biofuel cell
Grantee:Frank Nelson Crespilho
Support Opportunities: Regular Research Grants