Regulation of interleukin-6 and matrix metalloproteinases syntheses by bioflavonoids and photobiomodulation in human gingival fibroblasts

Author(s)

Cardoso, Lais Medeiros ; Pansani, Taisa Nogueira ; de Souza Costa, Carlos Alberto ; Basso, Fernanda Goncalves

• Document type: Journal article
• Source: Lasers in Medical Science; v. N/A, p. 15-pg., 2022-05-25.

Abstract

This study aimed to evaluate the separately effects of bioflavonoids proanthocyanidins, from grape seed extract (GSE) and synthetic naringenin (NA), as well as photobiomodulation (PBM) by low-level laser therapy on interleukin (IL)-6 and matrix metalloproteinases (MMPs) syntheses by human gingival fibroblasts (HGF). For this purpose, a connective tissue exposure (ulceration) model of HGF, stimulated with tumor necrosis factor-alpha (TNF-alpha), was used. Initially, the highest non-cytotoxic and non-genotoxic concentrations of bioflavonoids were determined by cell viability and micronuclei formation assays. Then, HGF were exposed to different stimuli: culture medium (negative control), dimethyl sulfoxide (DMSO), TNE-alpha, NA, GSE, TNF-alpha +NA, TNF-alpha +GSE, PBM (3 J/cm(2), 0.025 W, 780 nm), and TNF-alpha +PBM. Next, IL-6, MMP-2, and MMP-9 syntheses were assessed. The concentration of 10 mu g/mL of bioflavonoids increased cell viability at 24 and 48 h and did not present cytotoxic or genotoxic effects on HGF after 24, 48, and 72 h of contact. This concentration was selected for the assessment of bioflavonoids potential in modulating inflammatory mediators. TNF-alpha exposure enhanced IL-6 (170%), MMP-2 (10%), and MMP-9 (20%) syntheses, while a decrease of MMP-2 by 55% after exposure to TNF-alpha + GSE and 20% after TNF-alpha +NA and TNF-alpha +PBM was observed. MMP-9 synthesis was decreased by 35% after TNF-alpha +NA, 20% after TNF-alpha+GSE, and 30% after PBM. IL-6 was down-regulated by GSE in the presence of TNF-alpha (80%). In conclusion, TNF-alpha up-regulated IL-6 and MMPs, while bioflavonoids and PBM down-regulated MMP-2 and MMP-9 syntheses; GSE also decreased IL-6 synthesis, demonstrating the individual promising potential of these therapies for ulceration management. (AU)

• FAPESP's process: 18/11211-6 - BISPHOSPHONATES AND IMPLANTOLOGY: EFFECT OF METALLOPROTEINASE INHIBITORS ON IN VITRO MODEL
• Grantee: Fernanda Gonçalves Basso
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/16886-4 - Bisphosphonates and implantology: effect of metalloproteinases inhibitors in in vitro model
• Grantee: Laís Medeiros Cardoso
• Support Opportunities: Scholarships in Brazil - Doctorate