| Full text | |
| Author(s): |
Valerio, Hellen Paula
;
Ravagnani, Felipe Gustavo
;
Candela, Angela Paola Yaya
;
da Costa, Bruna Dias Carvalho
;
Ronsein, Graziella Eliza
;
Di Mascio, Paolo
Total Authors: 6
|
| Document type: | Journal article |
| Source: | ISCIENCE; v. 25, n. 4, p. 27-pg., 2022-04-15. |
| Abstract | |
The effects of UV light on the skin have been extensively investigated. However, systematic information about how the exposure to ultraviolet-A (UVA) light, the least energetic but the most abundant UV radiation reaching the Earth, shapes the subcellular organization of proteins is lacking. Using subcellular fractionation, mass-spectrometry-based proteomics, machine learning algorithms, immunofluorescence, and functional assays, we mapped the subcellular reorganization of the proteome of human keratinocytes in response to UVA light. Our workflow quantified and assigned subcellular localization for over 1,600 proteins, of which about 200 were found to redistribute upon UVA exposure. Reorganization of the proteome affected modulators of signaling pathways, cellular metabolism, and DNA damage response. Strikingly, mitochondria were identified as one of the main targets of UVA-induced stress. Further investigation demonstrated that UVA induces mitochondrial fragmentation, up-regulates redox-responsive proteins, and attenuates respiratory rates. These observations emphasize the role of this radiation as a potent metabolic stressor in the skin. (AU) | |
| FAPESP's process: | 15/07768-7 - Programmed cell death photoinduced mechanisms and different gene expression profiles |
| Grantee: | Felipe Gustavo Ravagnani |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 16/00696-3 - Proteomic as a tool to understand HDL function and composition |
| Grantee: | Graziella Eliza Ronsein |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 16/11430-4 - Proteome changes and DNA damage caused by photoinduced oxidative stress in cells |
| Grantee: | Hellen Paula Valerio |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 12/12663-1 - Singlet molecular oxygen and peroxides in chemical biology |
| Grantee: | Paolo Di Mascio |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 13/07937-8 - Redoxome - Redox Processes in Biomedicine |
| Grantee: | Ohara Augusto |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |