Highly effective Candida rugosa lipase immobilization on renewable carriers: Integrated drying and immobilization process to improve enzyme performance

Author(s)

Costa-Silva, T. A. ; Carvalho, A. K. F. ; Souza, C. R. F. ; Freitas, L. ; De Castro, H. F. ; Oliveira, W. P.

• Document type: Journal article
• Source: CHEMICAL ENGINEERING RESEARCH & DESIGN; v. 183, p. 15-pg., 2022-07-01.

Abstract

The overall stability of Candida rugosa lipase (CRL) was improved via immobilization onto low-cost eco-friendly carriers (agroindustrial wastes) followed by using spouted bed dryer or spray dryer. Lipase covalently immobilized on glutaraldehyde-activated rice husk proved the greatest performance, retaining 94.1% of the initial activity, followed by sugarcane bagasse (90.3%) and green coconut fiber (89.3%). On the other hand, the enzyme activity retention of CRL-lipase bioencapsulated in magnetic chitosan treated with glutaraldehyde or sodium tripolyphosphate ranged from 73.6% to 84.6%. The dried product moisture content, and water activities ranged from 4.1% and 6.5% and from 0.2 to 0.35, respectively. After storage for six months at 5 degrees C, the immobilized enzyme systems retained at least 70.0% of its initial activity (vs 18.3% retained activity for the free lipase). After ten reuse cycles, enzyme immobilized onto lignocellulosic carriers retained on average 72.7% of its initial activity. The thermostability of all immobilized derivatives was significantly improved and lipase immobilized onto rice husk showed a stabilization factor of 20.44 at 80 degrees C. The kinetic data showed that the CRL-lipase suffered alterations in catalytic behavior after the immobilization/drying, mainly a slight decrease in affinity to the substrate (up arrow KM). Among all the assessed immobilized lipase for aroma synthesis the rice husk immobilized lipase displayed the best result towards the production of isoamyl caprylate (62.40 g.L-1). In conclusion, this report reinforces the urgent need for the economic development of eco-sustainable immobilized biocatalysts to boost the use of enzymatic technology on industrial scale, and the feasibility of the association of immobilization and drying processes to improve the enzyme-quality features. (c) 2022 Institution of Chemical Engineers. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 11/00743-8 - Production and immobilization of lipases produced by the endophytic fungus Cercospora kikuchii for biotechnological applications
• Grantee: Tales Alexandre da Costa e Silva
• Support Opportunities: Scholarships in Brazil - Doctorate