| Full text | |
| Author(s): |
Vinicius Henrique Bello
[1]
;
Gabriel Madoglio Favara
[2]
;
Gustavo Verruma Bernardi
[3]
;
Jorge Alberto Marques Rezende
[4]
;
Renato Barbosa Salaroli
[5]
;
Elliot Watanabe Kitajima
[6]
Total Authors: 6
|
| Affiliation: | [1] Universidade de São Paulo. ESALQ. Depto. de Fitopatologia e Nematologia - Brasil
[2] Universidade de São Paulo. ESALQ. Depto. de Fitopatologia e Nematologia - Brasil
[3] Universidade de São Paulo. ESALQ. Depto. de Fitopatologia e Nematologia - Brasil
[4] Universidade de São Paulo. ESALQ. Depto. de Fitopatologia e Nematologia - Brasil
[5] Universidade de São Paulo. ESALQ. Depto. de Fitopatologia e Nematologia - Brasil
[6] Universidade de São Paulo. ESALQ. Depto. de Fitopatologia e Nematologia - Brasil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | Scientia Agricola; v. 80, 2022-09-12. |
| Abstract | |
ABSTRACT Sunflower (Helianthus annuus) plants showing symptoms of chlorosis, mosaic, chlorotic ringspot, and necrosis on younger leaves were found in a small experimental plot in Piracicaba, in the state of São Paulo, Brazil. Preliminary examinations by transmission electron microscopy of symptomatic leaf tissue revealed flexuous filamentous particles 13-15 nm wide and 700-750 nm long, and cytoplasmatic cylindrical inclusions typical of those found in plant cells infected by members of the Potyvirus genus. Total RNA extracted from symptomatic leaves and subjected to RT-PCR followed by partial nucleotide sequencing confirmed the presence of a potyvirus in the affected plants, which was identified as sunflower chlorotic mottle virus (SuCMoV), a member of the Sunflower chlorotic mottle virus (genus Potyvirus, family Potyviridae) species. Mechanical transmission assays with extracts of symptomatic sunflower leaves reproduced the original symptoms in sunflowers, mosaic symptoms in Zinnia elegans, and chlorotic local lesions in Chenopodium amaranticolor and C. quinoa. Sunflower and zinnia plants became infected after aphid transmission experiments with Myzus persicae. RT-PCR tests using specific primers for SuCMoV confirmed the presence of this virus in experimentally infected plants, meeting the criteria of Koch’s postulate. This is the first report of SuCMoV infecting sunflower plants in Brazil. (AU) | |
| FAPESP's process: | 18/01633-0 - Tomato severe rugose virus: potential of susceptible species as source of virus inoculum, vertical and horizontal transmission by B. tabaci MEAM1 and selection of mild strains for pre-immunization in tomato |
| Grantee: | Gabriel Madoglio Favara |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 21/03113-7 - On the possibility that phoresis is responsible for the dispersion of Brevipalpus mites and the viruses they transmit |
| Grantee: | Gustavo Verruma Bernardi |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 21/02179-4 - Evaluation of the diversity of molecular pathogens (virus and viroids) of plants in Brazi: Phase III - 2021/2023 |
| Grantee: | Elliot Watanabe Kitajima |
| Support Opportunities: | BIOTA-FAPESP Program - Regular Research Grants |
| FAPESP's process: | 20/05563-7 - The amplifier host hypothesis in the epidemiology of the Golden Mosaic of tomatoes, caused by Tomato Severe Rugose Virus (ToSRV): experimental proof and determination of the conditions of occurrence |
| Grantee: | Vinicius Henrique Bello |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/18274-3 - Begomovirus and crinivirus in solanasceous: regional molecular epidemiology and sustainable integrated management alternatives |
| Grantee: | Jorge Alberto Marques Rezende |
| Support Opportunities: | Research Projects - Thematic Grants |