Extraction optimization, biological activities, and application in O/W emulsion of deep eutectic solvents-based phenolic extracts from olive pomace

Author(s)

Pontes, Paula Virginia de Almeida ; Czaikoski, Aline ; Almeida, Naara Aparecida ; Fraga, Sara ; Rocha, Liliana de Oliveira ; Cunha, Rosiane Lopes ; Maximo, Guilherme Jose ; Batista, Eduardo Augusto Caldas

• Document type: Journal article
• Source: Food Research International; v. 161, p. 14-pg., 2022-08-11.

Abstract

This study evaluated the phenolic compound extraction from olive pomace with deep eutectic solvents (DES) prepared with choline chloride ([Ch]Cl) and four (poly-)carboxylic acids. Temperature, water addition in the solvent, and solid-liquid ratio were evaluate in total phenolic content and antioxidant activity of extracts ob-tained with DES and ethanol, as control. Moreover, the antimicrobial activities of solvents and extracts were evaluated. Oil-in-water emulsion with DES extract was prepared, characterized and its oxidative stability analyzed. The extract with the highest total phenolic content was obtained with [Ch]Cl:malonic acid. Under optimal conditions, DES extracted 9 % more total phenolic content than ethanol. DES extract showed superior antibacterial activity to the ethanolic extract, and its presence in oil-in-water emulsion increased the induction time in 10-fold when compared to the one prepared with water. These results reinforce that DES are a potential solvent for phenolic compound extraction from olive pomace with antibacterial and technological benefits. (AU)

• FAPESP's process: 17/04811-4 - Population structure of the Fusarium graminearum species complex and mycotoxin contamination of cultivated and native barley in Brazil
• Grantee: Liliana de Oliveira Rocha
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 14/21252-0 - Equilibrium and production processes of biofuels and bioproducts
• Grantee: Antonio José de Almeida Meirelles
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/08566-1 - Phase equilibrium thermodynamics for the improvement of physical properties of food bioproducts and their prediction in industrial and digestive processes
• Grantee: Guilherme José Maximo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/06134-4 - Multi-User Equipment approved in grant 2014/03002-7: cell imaging multi-mode reader
• Grantee: Marcelo Bispo de Jesus
• Support Opportunities: Multi-user Equipment Program