Conducting polymers modulating the catalytic activity of urease in thin composite films

Author(s)

de Jesus, Cleber Gomes ; Rodrigues, Rebeca da Rocha ; Caseli, Luciano ; Peres, Laura Oliveira

• Document type: Journal article
• Source: COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS; v. 654, p. 7-pg., 2022-09-13.

Abstract

Enzymes immobilized in matrices like conducting polymers (CPs) can form a supramolecular structure with enhanced biosensing performance, which depend on the structural polymer properties. This present paper employed CPs from the classes of thiophenes, fluorenes, phenylenes, and phenylene-vinylenes to immobilize urease as thin films and to investigate the relationship between the film structure and the enzyme activity. The films were assembled by the spin coating technique and characterized by UV-vis, fluorescence, and infrared spectroscopies. The enzymatic activity was estimated with UV-vis spectroscopy. Films containing different CPs presented specific enzyme activities dependent on polymers' size and polydispersity, but these activities were independent of their specific conductivity. Poly (9,9-dioctylfluorene-co-phenylene) (PFPh) presented the lower KM constant (1.52 mM.s(-1)) and was the polymer with lower molecular weight and polydispersity. The results suggested that small and homogeneous size chains increase the enzyme activity, which can be beneficial, generally speaking, for the construction of based-enzyme biosensors with enhanced efficiency. (AU)

• FAPESP's process: 20/04427-2 - Influence of conjugated polymers in biossensor for urea detection
• Grantee: Cléber Gomes de Jesus
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 19/03239-0 - Nanostructured interfaces for the investigation of bioactive substances in cell membrane models and for the construction of optoelectronic devices
• Grantee: Luciano Caseli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/50869-6 - INCT 2014: on Organic Electronics
• Grantee: Roberto Mendonça Faria
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants