| Full text | |
| Author(s): |
Alexander Bruck, Stefan
;
Contato, Alex Graca
;
Gamboa-Trujillo, Paul
;
de Oliveira, Tassio Brito
;
Cereia, Mariana
;
de Moraes Polizeli, Maria de Lourdes Teixeira
Total Authors: 6
|
| Document type: | Journal article |
| Source: | MICROORGANISMS; v. 10, n. 2, p. 15-pg., 2022-02-01. |
| Abstract | |
The isolation of filamentous fungal strains from remote habitats with extreme climatic conditions has led to the discovery of a series of enzymes with attractive properties that can be useful in various industrial applications. Among these, cold-adapted enzymes from fungi with psychrotrophic lifestyles are valuable agents in industrial processes aiming towards energy reduction. Out of eight strains isolated from soil of the paramo highlands of Ecuador, three were selected for further experimentation and identified as Cladosporium michoacanense, Cladosporium sp. (cladosporioides complex), and Didymella sp., this last being reported for the first time in this area. The secretion of seven enzymes, namely, endoglucanase, exoglucanase, beta -D-glucosidase, endo-1,4-beta -xylanase, beta -D-xylosidase, acid, and alkaline phosphatases, were analyzed under agitation and static conditions optimized for the growth period and incubation temperature. Cladosporium strains under agitation as well as incubation for 72 h mostly showed the substantial activation for endoglucanase reaching up to 4563 mU/mL and xylanase up to 3036 mU/mL. Meanwhile, other enzymatic levels varied enormously depending on growth and temperature. Didymella sp. showed the most robust activation at 8 degrees C for endoglucanase, beta -D-glucosidase, and xylanase, indicating an interesting profile for applications such as bioremediation and wastewater treatment processes under cold climatic conditions. (AU) | |
| FAPESP's process: | 18/07522-6 - Development of eco-friendly technologies for the hydrolysis of biomass and residues of cellulose pulp and paper industry |
| Grantee: | Maria de Lourdes Teixeira de Moraes Polizeli |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 08/57908-6 - National Institute of Science and Technology of Bioethanol |
| Grantee: | Marcos Silveira Buckeridge |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Thematic Grants |
| FAPESP's process: | 17/25862-6 - Structural studies and functional properties of xyloglucanases and lichenases: applications on hydrolysis of lignocellulosic biomass |
| Grantee: | Alex Graça Contato |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 17/09000-4 - Effects of temperature and water stress, in climate change scenario, on the fungal community in tropical soil and selection of nitrous oxide producing strains |
| Grantee: | Tássio Brito de Oliveira |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 14/50884-5 - INCT 2014: National Institute of Science and Technology of Bioethanol |
| Grantee: | Marcos Silveira Buckeridge |
| Support Opportunities: | Research Projects - Thematic Grants |